半定量 RT-PCR,RQRT-PCR,在线英语词典,英文翻译,专业英语

1) RQRT-PCR

半定量 RT-PCR

In this paper,we aimed to identify and characterize the G-protein β subunit from English grain aphid,Sitobion avenae by RT-PCR and (3 /5 )-RACE technique,and tissue specific expression was deter- mined by relative quantitative RT-PCR (RQRT-PCR).

根据所有已知 G 蛋白β亚基的保守区设计简并性寡核苷酸引物,通过3′-和5′-RACE方法结合 RT-PCR 技术,从麦长管蚜中分离全长 cDNA 序列,并用半定量 RT-PCR 的方法研究基因在不同组织的转录水平。

2) semi-quantity RT-PCR

半定量RT-PCR

In the present study,a semi-quantity RT-PCR for detection of abundance of liver PEPCK-C mRNA in peripartum dairy cows was established.

建立了奶牛肝PEPCK-C mRNA丰度的半定量RT-PCR检测方法,其优化的PCR反应条件(25μL)为:57℃退火、2。

Material and Methods By RT-PCR(semi-quantity RT-PCR,SQ-PCR), hOCT1 gene mRNA levels were detected from 9 couples of (18) samples.

对象及方法：采用半定量RT-PCR(semi-quantity RT-PCR，SQ-PCR)方法对服用伊马替尼慢性粒细胞白血病患者缓解或耐药前后的2个不同时点的9对(18份)标本进行了hOCT 1基因mRNA水平检测，并对SQ-PCR结果结合临床表现、实验室检查和细胞遗传学结果，分析其水平变化和与疾病缓解或伊马替尼耐药相关性。

3) semi-quantitative RT-PCR

半定量RT-PCR

Expression of miR393 in Leaves and Panicles at the Booting Stage of Rice under Drought Stress with Semi-Quantitative RT-PCR;

半定量RT-PCR方法研究microRNA393在旱处理后的孕穗期水稻叶片和穗部的表达情况

Establishment of the semi-quantitative RT-PCR system of nonexpressor of pathogenesis-related genes 1(NPR1) gene in sweet potato(Ipomoea batatas Lam.);

甘薯NPR1基因半定量RT-PCR检测方法的建立

Aplication of Semi-Quantitative RT-PCR in Analysis Gene Expression Level;

半定量RT-PCR法在检测基因表达水平中的应用

4) Semiquantitative RT-PCR

半定量RT-PCR

The results analysed by semiquantitative RT-PCR showed that MxFRO was induced by iron-deficiency stress in roots and leaves.

半定量RT-PCR结果表明MxFRO在小金海棠的根和叶中均受缺铁胁迫诱导。

The Establishment of A Methodology of Semiquantitative RT-PCR with Time-sharing Synchronized Circulation for Detection of E-cadherin Levels in Gastric Cancer Cells;

目的:探索建立分时同步循环法分管半定量RT-PCR体系,并检测不同分化程度胃癌细胞中E-钙粘附素的相对表达量。

Semiquantitative RT-PCR analysis of apoptotic protease activating factor 1 in oral squamous cell carcinoma;

方法:用半定量RT-PCR的方法检测18例正常口腔黏膜组织和32例OSCC组织中APAF1的表达情况。

5) semi-quantitive RT-PCR

半定量RT-PCR

The housefly larvae was induced by 30% H2O2 for 90 minutes,and the expression of antibacterial peptide genes of cecropin,defensin,diptericin,attacin,and lysozyme in transcription level was carried out on semi-quantitive RT-PCR.

选择30%H2O2诱导家蝇幼虫90 min,应用半定量RT-PCR的方法,以组成型表达的β-actin基因作为内参照,对抗菌活性物质基因cecropin,defensin,dipteri-cin,attacin和溶菌酶基因lysozyme在诱导后的表达情况进行了初步探索。

6) RT-PCR

RT-PCR半定量

参考词条

半定量RT-PCR方法半定量RT-PCR技术半定量尺RT-PCR 半定量逆转录聚合酶链式反应（RT-PCR）半定量逆转录聚合酶链反应技术（RT-PCR）半定量逆转录-聚合酶链反应（RT-PCR）半定量逆转录聚合酶链反应（RT-PCR）半定量逆转录聚合酶链反应（semi-quantitative RT-PCR） RT-PCR RT_PCR （RT）PCR RTPCR RT/PCR 实时RT-PCR 深圳香荚兰软件主体

补充资料：半定量分析

　　准确性比定量分析稍差的分析方法，特点是简单、迅速、费用低。常用于以下几种情况：①希望得知成分的大致含量，以便进一步选择合适的精确定量分析方法；②只要求分析快，不太追求成分的准确含量，例如某种合金型号的确定、工业生产中的中间控制、法医学中对毒物是否超过致死量的鉴定、试剂中杂质是否超过了法定标准的分析；③试样较少，没有理想的定量方法可采用。
　　

说明：补充资料仅用于学习参考，请勿用于其它任何用途。

首页 \| 翻译 \| 影视 \| 论坛 \| 索引荧光半定量RT-PCR RT-PCR半定量法

1) RQRT-PCR 半定量 RT-PCR 1. In this paper,we aimed to identify and characterize the G-protein β subunit from English grain aphid,Sitobion avenae by RT-PCR and (3 /5 )-RACE technique,and tissue specific expression was deter- mined by relative quantitative RT-PCR (RQRT-PCR). 根据所有已知 G 蛋白β亚基的保守区设计简并性寡核苷酸引物,通过3′-和5′-RACE方法结合 RT-PCR 技术,从麦长管蚜中分离全长 cDNA 序列,并用半定量 RT-PCR 的方法研究基因在不同组织的转录水平。 2) semi-quantity RT-PCR 半定量RT-PCR 1. In the present study,a semi-quantity RT-PCR for detection of abundance of liver PEPCK-C mRNA in peripartum dairy cows was established. 建立了奶牛肝PEPCK-C mRNA丰度的半定量RT-PCR检测方法,其优化的PCR反应条件(25μL)为:57℃退火、2。 2. Material and Methods By RT-PCR(semi-quantity RT-PCR,SQ-PCR), hOCT1 gene mRNA levels were detected from 9 couples of (18) samples. 对象及方法：采用半定量RT-PCR(semi-quantity RT-PCR，SQ-PCR)方法对服用伊马替尼慢性粒细胞白血病患者缓解或耐药前后的2个不同时点的9对(18份)标本进行了hOCT 1基因mRNA水平检测，并对SQ-PCR结果结合临床表现、实验室检查和细胞遗传学结果，分析其水平变化和与疾病缓解或伊马替尼耐药相关性。 3) semi-quantitative RT-PCR 半定量RT-PCR 1. Expression of miR393 in Leaves and Panicles at the Booting Stage of Rice under Drought Stress with Semi-Quantitative RT-PCR; 半定量RT-PCR方法研究microRNA393在旱处理后的孕穗期水稻叶片和穗部的表达情况 2. Establishment of the semi-quantitative RT-PCR system of nonexpressor of pathogenesis-related genes 1(NPR1) gene in sweet potato(Ipomoea batatas Lam.); 甘薯NPR1基因半定量RT-PCR检测方法的建立 3. Aplication of Semi-Quantitative RT-PCR in Analysis Gene Expression Level; 半定量RT-PCR法在检测基因表达水平中的应用 4) Semiquantitative RT-PCR 半定量RT-PCR 1. The results analysed by semiquantitative RT-PCR showed that MxFRO was induced by iron-deficiency stress in roots and leaves. 半定量RT-PCR结果表明MxFRO在小金海棠的根和叶中均受缺铁胁迫诱导。 2. The Establishment of A Methodology of Semiquantitative RT-PCR with Time-sharing Synchronized Circulation for Detection of E-cadherin Levels in Gastric Cancer Cells; 目的:探索建立分时同步循环法分管半定量RT-PCR体系,并检测不同分化程度胃癌细胞中E-钙粘附素的相对表达量。 3. Semiquantitative RT-PCR analysis of apoptotic protease activating factor 1 in oral squamous cell carcinoma; 方法:用半定量RT-PCR的方法检测18例正常口腔黏膜组织和32例OSCC组织中APAF1的表达情况。 5) semi-quantitive RT-PCR 半定量RT-PCR 1. The housefly larvae was induced by 30% H2O2 for 90 minutes,and the expression of antibacterial peptide genes of cecropin,defensin,diptericin,attacin,and lysozyme in transcription level was carried out on semi-quantitive RT-PCR. 选择30%H2O2诱导家蝇幼虫90 min,应用半定量RT-PCR的方法,以组成型表达的β-actin基因作为内参照,对抗菌活性物质基因cecropin,defensin,dipteri-cin,attacin和溶菌酶基因lysozyme在诱导后的表达情况进行了初步探索。 6) RT-PCR RT-PCR半定量参考词条半定量RT-PCR方法半定量RT-PCR技术半定量尺RT-PCR 半定量逆转录聚合酶链式反应（RT-PCR）半定量逆转录聚合酶链反应技术（RT-PCR）半定量逆转录-聚合酶链反应（RT-PCR）半定量逆转录聚合酶链反应（RT-PCR）半定量逆转录聚合酶链反应（semi-quantitative RT-PCR） RT-PCR RT_PCR （RT）PCR RTPCR RT/PCR 实时RT-PCR 深圳香荚兰软件主体补充资料：半定量分析　　准确性比定量分析稍差的分析方法，特点是简单、迅速、费用低。常用于以下几种情况：①希望得知成分的大致含量，以便进一步选择合适的精确定量分析方法；②只要求分析快，不太追求成分的准确含量，例如某种合金型号的确定、工业生产中的中间控制、法医学中对毒物是否超过致死量的鉴定、试剂中杂质是否超过了法定标准的分析；③试样较少，没有理想的定量方法可采用。　　说明：补充资料仅用于学习参考，请勿用于其它任何用途。
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