1) RT-PCR semi-quantity method
RT-PCR半定量法
2) semi-quantity RT-PCR
半定量RT-PCR
1.
In the present study,a semi-quantity RT-PCR for detection of abundance of liver PEPCK-C mRNA in peripartum dairy cows was established.
建立了奶牛肝PEPCK-C mRNA丰度的半定量RT-PCR检测方法,其优化的PCR反应条件(25μL)为:57℃退火、2。
2.
Material and Methods By RT-PCR(semi-quantity RT-PCR,SQ-PCR), hOCT1 gene mRNA levels were detected from 9 couples of (18) samples.
对象及方法：采用半定量RT-PCR(semi-quantity RT-PCR，SQ-PCR)方法对服用伊马替尼慢性粒细胞白血病患者缓解或耐药前后的2个不同时点的9对(18份)标本进行了hOCT 1基因mRNA水平检测，并对SQ-PCR结果结合临床表现、实验室检查和细胞遗传学结果，分析其水平变化和与疾病缓解或伊马替尼耐药相关性。
3.
Expression analysis of CHI of Kyoho grape was carried out by semi-quantity RT-PCR, using grape actin gene as an internal control.
试验从一年生巨峰扦插苗中克隆了CHI基因,并采用半定量RT-PCR技术,以actin基因为内参,分析了CHI基因在巨峰葡萄果实不同发育阶段及组织的表达。
3) semi-quantitative RT-PCR
半定量RT-PCR
1.
Expression of miR393 in Leaves and Panicles at the Booting Stage of Rice under Drought Stress with Semi-Quantitative RT-PCR;
半定量RT-PCR方法研究microRNA393在旱处理后的孕穗期水稻叶片和穗部的表达情况
2.
Establishment of the semi-quantitative RT-PCR system of nonexpressor of pathogenesis-related genes 1(NPR1) gene in sweet potato(Ipomoea batatas Lam.);
甘薯NPR1基因半定量RT-PCR检测方法的建立
3.
Aplication of Semi-Quantitative RT-PCR in Analysis Gene Expression Level;
半定量RT-PCR法在检测基因表达水平中的应用
4) Semiquantitative RT-PCR
半定量RT-PCR
1.
The results analysed by semiquantitative RT-PCR showed that MxFRO was induced by iron-deficiency stress in roots and leaves.
半定量RT-PCR结果表明MxFRO在小金海棠的根和叶中均受缺铁胁迫诱导。
2.
The Establishment of A Methodology of Semiquantitative RT-PCR with Time-sharing Synchronized Circulation for Detection of E-cadherin Levels in Gastric Cancer Cells;
目的:探索建立分时同步循环法分管半定量RT-PCR体系,并检测不同分化程度胃癌细胞中E-钙粘附素的相对表达量。
3.
Semiquantitative RT-PCR analysis of apoptotic protease activating factor 1 in oral squamous cell carcinoma;
方法:用半定量RT-PCR的方法检测18例正常口腔黏膜组织和32例OSCC组织中APAF1的表达情况。
5) semi-quantitive RT-PCR
半定量RT-PCR
1.
The housefly larvae was induced by 30% H2O2 for 90 minutes,and the expression of antibacterial peptide genes of cecropin,defensin,diptericin,attacin,and lysozyme in transcription level was carried out on semi-quantitive RT-PCR.
选择30%H2O2诱导家蝇幼虫90 min,应用半定量RT-PCR的方法,以组成型表达的β-actin基因作为内参照,对抗菌活性物质基因cecropin,defensin,dipteri-cin,attacin和溶菌酶基因lysozyme在诱导后的表达情况进行了初步探索。
2.
13 differential expression fragments were confirmed by semi-quantitive RT-PCR at different inoculating times.
对13个TDFs基因进行了半定量RT-PCR分析,结果表明13个差异片段在对照与处理,或是处理的不同时段存在着表达量的差异。
6) semi-quantitative fluorescent RT-PCR
荧光半定量RT-PCR
1.
Detection of deltamethrin resistance in Culex pipiens pallen with semi-quantitative fluorescent RT-PCR assay;
用荧光半定量RT-PCR检测蚊溴氰菊酯抗性
补充资料：半定量分析
准确性比定量分析稍差的分析方法，特点是简单、迅速、费用低。常用于以下几种情况：①希望得知成分的大致含量，以便进一步选择合适的精确定量分析方法；②只要求分析快，不太追求成分的准确含量，例如某种合金型号的确定、工业生产中的中间控制、法医学中对毒物是否超过致死量的鉴定、试剂中杂质是否超过了法定标准的分析；③试样较少，没有理想的定量方法可采用。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条