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1)  long RT-PCR
长链RT-PCR
1.
Amplification of complete genome of dengue 2 virus by long RT-PCR;
登革2型病毒全长基因组的长链RT-PCR法扩增
2.
Amplification of full-length cDNA of Japanese encephalitis virus WHe strain by long RT-PCR;
日本脑炎病毒WHe株全长cDNA的长链RT-PCR法的扩增
2)  Long RT PCR
长RT-PCR
3)  ICC/ stand-specific RT-PCR
链特异性ICC/RT-PCR
4)  reverse transcription-polymerase chain reaction
RT-PCR
1.
With beta-actin gene as the internal standard,reverse transcription-polymerase chain reaction(RT-PCR) was used to examine the expression of fms-like tyrosine kinase(Flt-1) mRNA in endometrium of Small Tail Han sheep and Northeast Fine-wool sheep.
以β-actin基因作为内源性内标,采用RT-PCR方法检测了Flt-1基因mRNA在东北细毛羊、小尾寒羊妊娠期不同阶段子宫内膜中的表达量。
2.
MethodsThe expression of FEZ1 mRNA in 50 cases of esophageal carcinoma and 50 cases of normal esophageal mucoma were detected by reverse transcription-polymerase chain reaction(RT-PCR).
方法采用RT-PCR法检测50例食管鳞癌及50例正常食管组织中FEZ1 mRNA的表达情况,分析其与食管鳞癌患者各临床病理指标的关系。
3.
Methods The mRNA of Ki-67 and VEGF were detected using reverse transcription-polymerase chain reaction(RT-PCR) in 55 RCC tissues and 20 normal renal tissues.
方法应用半定量RT-PCR技术检测55例肾癌组织和20例正常肾组织Ki-67和VEGFmRNA表达情况。
5)  Reverse transcription polymerase chain reaction
RT-PCR
1.
Then the level of VEGF mRNA in kidney was determined by reverse transcription polymerase chain reaction (RT-PCR); the expression of VEGF and VEGF.
【方法】取3月龄、12月龄大鼠作为对照组,24月龄大鼠作为观察组各6只,提取其肾脏组织的RNA,应用逆转录聚合酶链反应技术(RT-PCR)检测肾脏组织内VEGFmRNA转录水平,以及应用免疫组化技术检测肾小球VEGF和VEGFR蛋白的表达;应用酶联免疫法检测肾组织培养液的VEGF总含量。
2.
Methods Reverse transcription polymerase chain reaction (RT PCR) was used to evaluate the amount of insulin like growth factor mRNA in exercise induced damaged skeletal muscle, and specimens were used for ultrastructural observation.
方法利用IGF引物对不同损伤程度骨骼肌标本进行RT-PCR检测,同时观查损伤骨骼肌超微结构改变。
3.
Methods NIT-1 cells were exposed to cyclosporin A (10 μmol/L) for 24 and 48 h respectively, after which the amount of insulin release was determined by means of radioimmunoassay (RIA), and the differential expressions of Nuox23, Cox7c and Atp5K genes assessed by semi-quantitative reverse transcription polymerase chain reaction.
放射免疫测定法(RIA)检测胰岛素释放,半定量RT-PCR检测CsA处理NIT-1胰岛β细胞后线粒体氧化磷酸化酶系中的几种主要成员(Nuox23、Cox7c和Atp5K)在mRNA水平上的表达。
6)  RTPCR
RT-PCR
1.
Expression of cbfa1 gene in rat osteosarcoma UMR106 cell was detected by RTPCR after the 72?h s treatment of FSK88 liquid with different mol concentration(25,50,100?μmol/L),Retinoic Acid(RA,10?μmol/L) and mixed liquid of FSK88 and RA(1∶1).
以大鼠成骨肉瘤细胞———UMR106为模型,应用RT-PCR方法,检测用不同浓度的FSK88药液(25,50,100μmol/L)、维甲酸(RA,10μmol/L,阳性对照)以及FSK88+RA(等体积)混合药液处理细胞72 h后,细胞内cbfa1基因表达量的变化。
2.
by RTPCR and 3′-RACE technology.
]实生苗为材料,采用RT-PCR及RACE技术,获得了PPF-1(开花、衰老相关基因)同源基因的cDNA全长序列PtPPF-1,该cDNA全长1 493 bp,编码452个氨基酸,与豌豆PPF-1、拟南芥ALB3、水稻PPF-1及葡萄一未命名基因的氨基酸序列同源性较高,说明PPF-1基因在进化过程中可能变异较小。
补充资料:脂肪乳剂(中链及长链复合剂) (10%~20%中/长链脂肪乳剂)
药物名称:脂肪乳剂(中链和长链复合物)

英文名:Fat Emulsion for Injection(MCT/LCT)

别名: 脂肪乳剂(中链及长链复合剂) (10%~20%中/长链脂肪乳剂)
外文名:FatEmulsion forInjection(MCT/LCT)
药理作用及用途:
胃肠外给药,可补充人体热能,避免必需脂肪酸缺乏。具有易于氧化水解、氧化完全、生酮作用和对肝功能影响较少等特点。
适用于创伤以及肝功能不全病人。
用法及用量: 同长链脂肪乳剂。
不良反应: 基本同长链脂肪乳剂。
注意事项: 基本同长链脂肪乳剂。
规格: 注射乳剂 250ml, 500ml



类别:肠内、肠外营养药
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条