1) strand-specific real-time RT-PCR
链特异性荧光定量RT-PCR
1.
The methods of MTT,fluorescence staining of Hoechst 33258,TdT-mediated dUTP nick-end-labeling(TUNEL),flow cytometry and strand-specific real-time RT-PCR were used to assay the inhibitory effects of FMDV on the growth of BHK-21 cells,the morphological features of apoptotic cells,the apoptosis apex,the cell cycle and the RNA product of replication system in cells respectively.
试验利用口蹄疫病毒感染BHK-21细胞,通过MTT法、Hoechst 33258染色、原位末端标记技术(TUNEL)、流式细胞术和链特异性荧光定量RT-PCR,分别就口蹄疫病毒对BHK-21细胞生长的抑制作用、凋亡细胞的形态学和分子生物学特征、凋亡峰的出现和细胞周期的变化以及口蹄疫病毒基因组在BHK-21细胞内的复制情况进行了检测。
2) semi-quantitative fluorescent RT-PCR
荧光半定量RT-PCR
1.
Detection of deltamethrin resistance in Culex pipiens pallen with semi-quantitative fluorescent RT-PCR assay;
用荧光半定量RT-PCR检测蚊溴氰菊酯抗性
3) Fluorescence quantitative RT-PCR
荧光定量RT-PCR
1.
Rapid detection of influenza virus A by fluorescence quantitative RT-PCR;
荧光定量RT-PCR快速检测A型流感病毒
2.
Development of fluorescence quantitative RT-PCR for detection of hsp_(70) and hsp_(90) mRNA from pigs;
猪hsp_(70)和hsp_(90)荧光定量RT-PCR检测方法的建立
3.
Detection of Citrus tristeza virus using conventional and fluorescence quantitative RT-PCR assays;
应用常规RT-PCR和荧光定量RT-PCR检测柑桔衰退病毒
5) Real-time RT-PCR
荧光定量RT-PCR
1.
Rapid detection of enterovirus with TaqMan real-time RT-PCR;
肠道病毒TaqMan荧光定量RT-PCR法快速检测
2.
Development of a SYBR GreenⅠ Real-time RT-PCR assay for detection of canine distemper virus;
SYBR GreenⅠ荧光定量RT-PCR检测犬瘟热病毒方法的建立及应用
3.
Detection of classical swine fever virus by real-time RT-PCR;
用荧光定量RT-PCR方法检测猪瘟病毒
6) Fluorescent Quantitative RT-PCR
荧光定量RT-PCR
1.
Development of real-time fluorescent quantitative RT-PCR for detection of avian reovirus;
禽呼肠孤病毒实时荧光定量RT-PCR检测方法的建立
2.
Development of a fluorescent quantitative RT-PCR assay for detection of expression of leptin mRNA;
乳牛瘦蛋白基因荧光定量RT-PCR检测方法的建立
3.
Development of a TaqMan fluorescent quantitative RT-PCR assay for detection of porcine IFN-γ mRNA
猪IFN-γ mRNA Taq Man荧光定量RT-PCR检测方法的建立
补充资料：周期性特异性药物
周期性特异性药物
仅能杀灭*细胞增殖周期中某一期癌细胞的抗癌药物。依其作用又分为两类：①作用于S期药物，如*羟基脲、*阿糖胞苷，甲氨蝶呤、巯基嘌呤、硫鸟嘌呤等。其中前两种药物特异性较高，主要干扰DNA合成，但因也能干扰 RNA的合成，可能起到一定的自限作用。临床上宜缓慢静滴或肌注给药。②作用于M期的药物，如长春新碱等。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条