1)  Western-blotting
Western-blotting
1.
Methods Ion exchange chromatography,gel filtration,SDS-PAGE and Western-blotting technique were used to identify and partially purify the allergens from the crude silkworm pupa extract.
方法采用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)和Western-blotting,鉴定了家蚕蚕蛹粗浸液中变应原蛋白质分子,并通过阴离子交换和凝胶过滤层析分别对家蚕蚕蛹变应原蛋白质进行初步纯化。
2.
To extract,isolate and identify the main protein allergens from wheat(Triticum aestivum)and buckwheat(Fagopyrum esculentum),the total protein from wheat and buckwheat were extract by PBS,the allergens were identified with 10 allergic patients serum by Western-Blotting after SDS-PAGE.
为了提取、分离小麦(Triticum aestivum)和荞麦(Fagopyrum esculentum)的主要过敏原并对其免疫学特性进行鉴定,以磷酸盐缓冲液(PBS)制备小麦和荞麦总蛋白粗提液,通过SDS-PAGE分析总蛋白组成,利用麦类食品过敏患者血清中的IgE能特异性结合过敏原的特性,通过免疫印迹(Western-Blotting)鉴定其中的过敏原成分,然后用离子交换层析(DE-52)对总蛋白进行初步分离,并用Western-Blotting鉴定分离后组份的免疫学特性。
2)  Western-blotting
免疫印迹技术
3)  Western-blotting
蛋白质-印迹
1.
The primary and secondary allergen proteins were identified by Western-blotting.
[方法]用Coca's液提取法制备龙虾变应原粗浸液,经聚丙烯酰胺凝胶电泳(SDS-PAGE)分离蛋白质组分,并用凝胶成像系统测定各组分的相对分子质量;采用蛋白质-印迹(Western-blotting)鉴定龙虾的主要及次要变应原。
4)  Western Blotting
Western Blotting法
1.
The content of P53 protein in their peripheral blood was detected by Western Blotting assay.
方法选取35名阳江天然放射高本底地区(HBRA)男性居民作为暴露组,邻近地区(CA)男性居民35名作为对照组,应用Western Blotting法测定两组人群外周血中P53蛋白含量。
5)  Western Blotting
Western Blotting
1.
Development of Western blotting for detecting BSE and scrapie;
疯牛病和羊痒病Western blotting检测方法的建立
2.
Methods Western blotting and immunofluorescence double staining methods were used.
方法Western blotting和免疫荧光双重染色。
3.
Western blotting was used to detect Notch1 in the transfected cells,parental cells,and empty vector-tra.
1-Notch1,转染EC9706细胞,利用Western blotting检测转染pcDNA3。
6)  Western blotting
Western印迹法
1.
Using western blotting and MTT chemosensi-tive testing, the relationship between the expression of NFkB family proteins, P53 and MDM2 in ovarian cancer cell lines and chemotherapy resistance of ovarian cell was ob.
培养13个卵巢癌细胞系,提取癌细胞蛋白,采用Western印迹法观察NFkB家族蛋白、P53和MDM2的表达,并用四氮唑盐法药敏试验观察与卵巢癌细胞化疗耐药性关系。
2.
Using the mouse antibody to Hsp70of human,pig,dog,etc,Hsp70was detected from seven randomly sele cted yeast strains by Western blotting.
随机抽取其中7株新生隐球菌,采用鼠抗人、猪、狗等的Hsp70抗体,通过Western印迹法来检测其Hsp70,根据实验信号强弱定性判断在不同刺激条件下的Hsp70产量。
3.
Using Western blotting and MTT chemosensitive testing, the relationship between the expression of NFκB family proteins in ovarian cancer cell lines with chemotherapy resistance of ovarian cell was observed.
方法 :培养 13株卵巢癌细胞系 ,对其进行胞质、胞核蛋白质提取 ,Western印迹法观察NFκB家族蛋白及其抑制物的表达 ,并用MTT检测药敏、观察NFκB家族蛋白与卵巢癌细胞化疗耐药性关系。
参考词条
补充资料：blotting
分子式：
CAS号：

性质：方法是将层析或电泳分离后的DNA、RNA或蛋白质组分从支持物(如凝胶)上照原样转移到另一种支持物(如特种滤纸、薄膜)上，然后通过适当的探针进行鉴定，并可从中筛选出所需的单一组分。转移的方法可以依据毛细管作用，如DNA印迹转移(Southern blotting)、RNA印迹转移(Northern blotting)、蛋白质印迹(Western blot-ting)，亦可用电泳的方法，如电转移(electroblotting)

说明：补充资料仅用于学习参考，请勿用于其它任何用途。