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1)  Western blotting
蛋白质免疫印迹法
2)  Western blot
蛋白质免疫印迹
1.
METHODS: The amelogenin mRNA expression patterns were determined by the reverse transcription polymerase chain reaction (RT-PCR),and the protein expression was studied with Western blotting.
方法:采用反转录多聚酶链式反应(RT-PCR)技术,检测培养细胞中釉原蛋白mRNA的表达,采用蛋白质免疫印迹技术检测培养细胞中釉原蛋白的表达。
3)  Western Blot
免疫蛋白印迹法
1.
Methods Western blot and RT-PCR were employed to investigate the expression of hPR isoforms in ectopic lesion from adenomyosis and normal endometrium from controls.
方法运用免疫蛋白印迹法(Western blot)和RT-PCR方法测定子宫腺肌病患者(24例)病灶及其在位内膜及对照组(19例)子宫内膜标本中孕激素受体亚型在蛋白和mRNA水平的表达,计算机图像分析、半定量研究孕激素受体亚型的表达。
4)  Western blot
蛋白免疫印迹法
1.
Standard criteria of Western blot for the diagnosis of Lyme disease caused by Borrelia garinii in China;
中国莱姆病伽氏疏螺旋体蛋白免疫印迹法诊断标准的研究
2.
In order to search for the cross-reactive antigens that can be used in Western blot assay for the accurate diagnosis of patients with Lyme disease, the Chinese strain PD91 of Borrelia burgdorferi was used in Western blot assay as antigen to detect the presence of antibodies (IgG and IgM) in sera of patients with syphilis and leptospirosis.
目的研究莱姆病螺旋体与梅毒、钩端螺旋体较常出现的交叉反应抗原,明确产生交叉反应的蛋白抗原成分,为精确蛋白免疫印迹法检测莱姆病的阳性判断标准提供参考依据。
5)  Western blotting
蛋白免疫印迹法
1.
Methods: esophageal membranous epithelium were examined for E-Cadherin by Western blotting.
方法:采用蛋白免疫印迹法对各组食管黏膜上皮组织E-Cad表达进行研究。
6)  Western blot ELISA
蛋白质免疫印迹酶联免疫吸附法
1.
Methods:HSP 70 antibodies were examined by Western blot ELISA between 37 patients of Henoch-Schonlein purpura and 35 controls.
方法采用蛋白质免疫印迹酶联免疫吸附法(Western blot ELISA)测定37例病例组与35例对照组血浆中热休克蛋白70(HSP70)抗体。
补充资料:蛋白质印迹法
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CAS号:

性质:一种免疫测定微量蛋白质的技术。样品经SDS-聚丙烯酰胺凝胶电泳后,将分辨的蛋白质转移到硝酸纤维膜上,加入放射标记的抗体一起保温,进行抗原抗体结合反应。然后冲洗去掉未结合的抗体,将薄膜贴在X线底片上,通过放射自显影进行定位和检测。

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