1) Western blot
Western印迹
1.
coli, to identify the protein by Western blot and purify the protein for further investigation as tumor vaccine.
目的:利用大肠杆菌表达人CT抗原NY-ESO-1,对表达产物进行Western印迹鉴定和纯化,为今后利用其进行肿瘤疫苗的研究奠定基础。
2.
The expression of PPARγ, α-smooth muscle actin(α-SMA), and type Ⅰ and Ⅲ collagen was detected by means of RT-PCR, Western blot and immunocytoc.
方法 设立空白对照组、3μmol L罗格列酮组、10μmol L罗格列酮组;分别 用RT PCR、Western印迹、免疫细胞化学方法检测PPARγ的表达;用Western印迹和免疫细胞化学方法检测α 平滑肌 肌动蛋白(α SMA)及Ⅰ、Ⅲ型胶原的表达;四甲基偶氮唑盐(methythiazolyltetrazolium,MTT)法检测细胞增殖;流式细胞 仪分析细胞凋亡。
3.
Cancer cells in each frozen section were captured with the LCM method and processed for Western blot anal.
方法:应用免疫组织化学法对 135例卵巢癌组织进行EphA2检测,对其中15例不同级别EphA2蛋白表达的冰冻组织标本,应用激光捕获显微切 割技术(LCM)捕获卵巢癌细胞,应用Western印迹分析方法检测其蛋白表达水平。
2) Western blotting
Western印迹
1.
METHODS: The mRNA level and protein expression of ANXA1 in some OSCC cell lines and human primary OSCC tissue specimens were detected using real-time PCR ,Western blotting and immunohistochemistry.
方法:应用实时定量PCR、Western印迹方法和免疫组化方法检测ANXA1在口腔鳞癌细胞株和30例原发口腔鳞癌中的表达,采用SPSS10。
2.
Methods The ES antigens (ESA) of Trichinella spiralis muscle larvae cultured in vitro at 18 h and 30 h were analyzed by SDS-PAGE and Western blotting.
方法 应用SDS PAGE和Western印迹对旋毛虫肌幼虫体外培养 18、3 0h后的ES抗原中的蛋白组分进行研究。
3.
9 breast cancer cell lines were cultured and cells harvested, protein of p50, p65 and IkB extracted from cytoplasm, nuclear separately to detect the expression of p50, p65, IkB proteins by Western blotting.
用 L-1 5培养液培养并传代 9个乳腺癌细胞系 ,收获细胞 ,提取胞浆、胞核蛋白质 ,Western印迹法观察 NFk B家族蛋白及其抑制物的表达 ;采用 2 0 J· m- 2 剂量紫外线照射细胞 ,继续培养细胞并提取蛋白质进行检测。
3) Western blotting
Western印迹法
1.
Using western blotting and MTT chemosensi-tive testing, the relationship between the expression of NFkB family proteins, P53 and MDM2 in ovarian cancer cell lines and chemotherapy resistance of ovarian cell was ob.
培养13个卵巢癌细胞系,提取癌细胞蛋白,采用Western印迹法观察NFkB家族蛋白、P53和MDM2的表达,并用四氮唑盐法药敏试验观察与卵巢癌细胞化疗耐药性关系。
2.
Using the mouse antibody to Hsp70of human,pig,dog,etc,Hsp70was detected from seven randomly sele cted yeast strains by Western blotting.
随机抽取其中7株新生隐球菌,采用鼠抗人、猪、狗等的Hsp70抗体,通过Western印迹法来检测其Hsp70,根据实验信号强弱定性判断在不同刺激条件下的Hsp70产量。
3.
Using Western blotting and MTT chemosensitive testing, the relationship between the expression of NFκB family proteins in ovarian cancer cell lines with chemotherapy resistance of ovarian cell was observed.
方法 :培养 13株卵巢癌细胞系 ,对其进行胞质、胞核蛋白质提取 ,Western印迹法观察NFκB家族蛋白及其抑制物的表达 ,并用MTT检测药敏、观察NFκB家族蛋白与卵巢癌细胞化疗耐药性关系。
4) Western blot
Western印迹法
1.
Methods Western blot and immunohistochemistry were performed to detect the expression and phosphorylation status of GluR1.
方法采用Western印迹法、免疫组织化学法检测纹状体AMPA受体亚单位GluR1表达及磷酸化状态。
5) Western blot
Western免疫印迹
1.
Methods The expressions of haptoglobin protein and mRNA in the skin tissues of 30 patients with CA and 20 normal controls were detected by immunohistochemistry(IHC),Western blot,and hybridization in situ.
方法:采用原位杂交、免疫组织化学技术和Western免疫印迹的方法,检测30例尖锐湿疣患者皮损及20例正常包皮组织中结合珠蛋白mRNA及其蛋白的表达情况。
2.
Methods Western blot was performed to detect Nogo-A proteolytic fragment after spinal cord transection,brain ischemia and KA-induced cerebral cortex injury in rats.
方法 制作大鼠脊髓横断、脑缺血和KA注射所致的脑损伤模型 ,Western免疫印迹方法检测Nogo A降解片段。
3.
Methods:Samples were obtained from 30 CA patients and 20 controls,the expression of MCP-1 protein and mRNA were detected by hybridization in situ,immunohistochemistry and Western Blot.
方法:采用原位杂交、免疫组化和Western免疫印迹的方法,检测30例尖锐湿疣表皮及20例正常包皮表皮中MCP-1 mRNA及其蛋白的表达水平。
6) Western blot
Western印迹分析
1.
HSP72 mRNA and HSP70 expression in heart and vessel tissues of both simulated weightless and control rats exposed to heat stress (ambient temperature, Ta=43℃) and recovered at Ta of 25℃ for 1 h (CON H 1, SUS H 1) or 2 h (CON H 2, SUS H 2) were analyzed using Northern blot and Western blot.
用Northern杂交与Western印迹分析检测 4周模拟失重大鼠热应激后并在室温下恢复 1h (SUS H1)或 2h (SUS H2 )心肌、血管组织HSP70表达的变化。
补充资料：Western blotting
分子式：
CAS号：
性质：一种类似于DNA斑迹法，用于从凝胶中转移蛋白质的技术。转移到膜上的蛋白质用同位素或酶标记过的抗体显示目的带的存在。
CAS号：
性质：一种类似于DNA斑迹法，用于从凝胶中转移蛋白质的技术。转移到膜上的蛋白质用同位素或酶标记过的抗体显示目的带的存在。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条