1) Over-lapping extensive PCR
重叠引物PCR
2) gene slicing by overlap extension
重叠延伸引物PCR
3) Multiplex PCR
多重引物PCR
4) Multi-ASP-PCR
多重引物特异PCR
5) Overlapping PCR
重叠PCR
1.
The gene fragments of two tandems were synthesized by overlapping PCR,inserted into pQE-30 vector,and transformed in Escherichia coli.
对其基因进行优化后,经重叠PCR方法合成串联体A、B的全长基因。
2.
Objective: For expression in Aspergillus awamori,the plant sweet protein brazzein gene was synthesized with overlapping PCR.
目的:为在泡盛曲霉(Aspergillus awamori)中进行表达,采用重叠PCR合成了植物甜蛋白brazzein基因。
3.
Then with overlapping PCR technology to link this two DNA together to form a complete ZP 3 gene encoding.
再经重叠PCR技术将这两段DNA连接在一起,构成完整的ZP 3基因的编码区。
6) overlap PCR
重叠PCR
1.
Method In this study,the fusion gene ctxAB containing ctxA and ctxB gene was obtained from DNA of Vibrio cholera by overlap PCR,and to construct recombinant plasmid pET-ctxAB the fusion gene ctxAB was cloned into prokaryotic expressed vector pET32a(+) co.
方法以霍乱弧菌DNA为模板,利用重叠PCR技术,扩增出含有ctxA和ctxB的融合基因片段ctxAB,并与带有硫氧还蛋白(Trx)基因的高效原核表达质粒pET32a(+)定向重组,构建重组质粒,转化大肠杆菌BL21(DE3)。
2.
The human anti-HBsAg ScFv and interferon gamma were connected with the linker to construct the ScFv-IFNγ gene by using overlap PCR techniques.
采用重叠PCR技术 ,将人源性抗HBsAg单链抗体基因与干扰素 γ 基因用柔性肽段碱基连接成单一基因 。
3.
The H chain and L chain gene of anti_HBsAg fab were connected to construct single_chain fab gene using overlap PCR techniques.
采用重叠PCR技术 ,以抗乙肝表面抗原 (HBsAg)IgG铰链区基因为Linker将Fab抗体基因的重链和轻链连接起来 ,构成单链Fab基因。
补充资料：梅花引 中州乐府案此下中州乐府原有梅花引
【诗文】：
蒿火目。藜羹腹。书生宁有封侯骨。长须奴。下泽车。艰关险阻，谁教涉畏途。半生落漠长安道。一事无成双鬓老。南辕胡。北辕吴。功名富贵，情知不可图。槐安梦。鼓笛弄。驰骤百年尘一哄。陶渊明。张季鹰。一杯浊酒，焉知身後名。有溪可渔林可缴。须信在家贫也乐。熊门春。＊江云。几时作个，山间林下人。
【注释】：
【出处】：
蒿火目。藜羹腹。书生宁有封侯骨。长须奴。下泽车。艰关险阻，谁教涉畏途。半生落漠长安道。一事无成双鬓老。南辕胡。北辕吴。功名富贵，情知不可图。槐安梦。鼓笛弄。驰骤百年尘一哄。陶渊明。张季鹰。一杯浊酒，焉知身後名。有溪可渔林可缴。须信在家贫也乐。熊门春。＊江云。几时作个，山间林下人。
【注释】：
【出处】：
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条