1) overlap extension of PCR
套叠PCR
2) GeneSOEing PCR
套叠PCR肠激酶
3) Overlapping PCR
重叠PCR
1.
The gene fragments of two tandems were synthesized by overlapping PCR,inserted into pQE-30 vector,and transformed in Escherichia coli.
对其基因进行优化后,经重叠PCR方法合成串联体A、B的全长基因。
2.
Objective: For expression in Aspergillus awamori,the plant sweet protein brazzein gene was synthesized with overlapping PCR.
目的:为在泡盛曲霉(Aspergillus awamori)中进行表达,采用重叠PCR合成了植物甜蛋白brazzein基因。
3.
Then with overlapping PCR technology to link this two DNA together to form a complete ZP 3 gene encoding.
再经重叠PCR技术将这两段DNA连接在一起,构成完整的ZP 3基因的编码区。
4) overlap PCR
重叠PCR
1.
Method In this study,the fusion gene ctxAB containing ctxA and ctxB gene was obtained from DNA of Vibrio cholera by overlap PCR,and to construct recombinant plasmid pET-ctxAB the fusion gene ctxAB was cloned into prokaryotic expressed vector pET32a(+) co.
方法以霍乱弧菌DNA为模板,利用重叠PCR技术,扩增出含有ctxA和ctxB的融合基因片段ctxAB,并与带有硫氧还蛋白(Trx)基因的高效原核表达质粒pET32a(+)定向重组,构建重组质粒,转化大肠杆菌BL21(DE3)。
2.
The human anti-HBsAg ScFv and interferon gamma were connected with the linker to construct the ScFv-IFNγ gene by using overlap PCR techniques.
采用重叠PCR技术 ,将人源性抗HBsAg单链抗体基因与干扰素 γ 基因用柔性肽段碱基连接成单一基因 。
3.
The H chain and L chain gene of anti_HBsAg fab were connected to construct single_chain fab gene using overlap PCR techniques.
采用重叠PCR技术 ,以抗乙肝表面抗原 (HBsAg)IgG铰链区基因为Linker将Fab抗体基因的重链和轻链连接起来 ,构成单链Fab基因。
5) nested PCR
套式PCR
1.
Development of the nested PCR assay for detection of dairy cattle-derived Giardia lamblia;
牛源贾第虫套式PCR检测方法的建立
2.
Detection of SARS viruses in specimens from SARS patients by one-step and nested PCR assays;
应用一步和套式PCR法检测SARS患者标本中的SARS病毒核酸序列
3.
Study on detection methods of Chlamydia psittaci by nested PCR and DNA sequencing analysis;
鹦鹉热衣原体套式PCR和DNA测序方法研究
6) Nested-PCR
套式PCR
1.
Detection of Actinobacillus pleuropneumoniae by nested-PCR and real-time PCR;
套式PCR和实时荧光PCR在检测胸膜肺炎放线杆菌上的应用
2.
Research of Detection of Rabies by Nested-PCR;
狂犬病套式PCR检测技术的研究
3.
The detection of Actinobacillus pleuropneumoniae by nested-PCR;
猪传染性胸膜肺炎放线杆菌的套式PCR检测
补充资料：嵌套PCR
分子式：
CAS号：
性质：后一次PCR的引物互补于前一次PCR所扩增序列的内侧。
CAS号：
性质：后一次PCR的引物互补于前一次PCR所扩增序列的内侧。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条