1) Semi-nested RT-PCR
半套式RT-PCR
1.
According to the nucleotide sequence of the S1 gene of MDRV registered in GenBank,a set of primers C1、HP11 and HP12 were designed to amplify a specific 300 bp fragment by semi-nested RT-PCR.
参考GenBank中番鸭呼肠孤病毒(muscovy duck reovirus,MDRV)S1基因序列设计并合成3条引物C1、HP11和HP12,组成半套式RT-PCR,扩增片段为300 bp。
2.
A specific semi-nested RT-PCR based on the RNA-dependent RNA polymerase gene in AF469603 of Chinese bee (Apis sinensis) sacbrood virus was established to detect Chinese bee sacbrood virus.
基于中蜂囊状幼虫病病毒基因组序列AF469603中的RNA依赖的RNA聚合酶基因,建立该病毒的半套式RT-PCR检测方法。
3.
According to the nucleotide sequence of the U25631 gene of Salmonella registered in GeneBank, a set of primers were designed to amplify a specific 581 bp fragment by Semi-nested RT-PCR.
为建立一种快速、特异性和实用性强、灵敏度高的沙门氏菌检测方法,参考GeneBank中登录号为U25631的沙门氏菌基因序列,设计并合成引物,组成半套式RT-PCR,扩增片段为581bp。
2) Semi-nested PCR
半嵌套式RT-PCR
1.
The Study of WT1 Gene Expression in the Urine from Patients with Renal Diseases by Semi-nested PCR;
半嵌套式RT-PCR法检测肾病患者尿沉渣中WT1基因表达研究
3) clone and sequencing
RT-半嵌套式pcR
4) nested reverse transcription-polymerase chain reaction
套式RT-PCR
1.
Objective: To establish a nested reverse transcription-polymerase chain reaction (nested RT-PCR) assay for detectioning specific fragment of human Rhinovirus (HRV) RNA,and to analyze the sequence of amplicons.
研究目的： 建立套式逆转录聚合酶链反应(nested reverse transcription-polymerase chain reaction，nested RT-PCR)检测临床样本鼻病毒(human rhinovirus，HRV)基因的方法，并进一步对扩增产物进行序列分析；用套式RT-PCR及直接免疫荧光(direct immunofluorescence assay，DFA)的方法对包括HRV在内的引起小儿急性下呼吸道感染(acute low respiratory tract infection，ALRTI)的常见病毒病原进行检测，以了解上海地区ALRTI患儿病毒感染的现状。
5) Nested RT-PCR
套式RT-PCR
1.
Detection of classical swine fever virus RNA by nested RT-PCR from paraffin-embedded tissues;
用套式RT-PCR技术检测石蜡包埋组织中的猪瘟病毒RNA
2.
Development of Nested RT-PCR to Detect Rabies Virus and Its Application in Saliva of Dogs and Cats in Shanghai;
套式RT-PCR检测狂犬病毒方法的建立及其在上海地区犬、猫唾液样本的应用
3.
Development and Initial Application of nested RT-PCR Method for Japanese Encephalitis virus
日本脑炎病毒套式RT-PCR方法的建立与初步应用
6) nest RT-PCR
套式RT-PCR
1.
For each sample,RNA was extracted using commercial kit and7Nest RT-PCR using a14-pair different SARS-associated coronavirus(SARS-CoV)special primers were carried out simultaneously.
方法以2003年5月16日至23日在本院住院的46例临床确诊SARS患者为研究对象,连续留取粪便标本共103份,提取RNA后采用14对SARS-CoV特异性引物,对每份标本同时进行7次套式RT-PCR扩增,只要有1次RT-PCR结果阳性即判定该标本为阳性。
补充资料：嵌套式PCR
分子式：
CAS号：
性质：后一次PCR的引物互补于前一次PCR所扩增序列的内侧。
CAS号：
性质：后一次PCR的引物互补于前一次PCR所扩增序列的内侧。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条