1) semi-nested PCR
半套式PCR
1.
Detection of Coxiella burnetii by semi-nested PCR and DNA probe;
半套式PCR和DNA探针技术检测Q热立克次体
2.
To seek a rapid and valid PCR method for the detection of Theileria,we established two kinds of routine PCR for 18S rRNA gene and surface protein gene,respectively,and a semi-nested PCR for 18S rRNA gene.
为寻求快速、有效检测羊泰勒虫的PCR方法,本试验建立了检测羊泰勒虫18SrRNA基因和表面蛋白基因的两种常规PCR方法和一种检测18SrRNA基因的半套式PCR方法,并从其敏感性和临床样本检出率等方面进行了比较。
3) heminested PCR
半嵌套式PCR
1.
The heminested PCR results showed that the foreign genes of the seven positi.
半嵌套式PCR检测结果表明阳性样品中的外源目的基因为来自根癌农杆菌CP4菌株的EPSPS基因。
4) Semi-nested RT-PCR
半套式RT-PCR
1.
According to the nucleotide sequence of the S1 gene of MDRV registered in GenBank,a set of primers C1、HP11 and HP12 were designed to amplify a specific 300 bp fragment by semi-nested RT-PCR.
参考GenBank中番鸭呼肠孤病毒(muscovy duck reovirus,MDRV)S1基因序列设计并合成3条引物C1、HP11和HP12,组成半套式RT-PCR,扩增片段为300 bp。
2.
A specific semi-nested RT-PCR based on the RNA-dependent RNA polymerase gene in AF469603 of Chinese bee (Apis sinensis) sacbrood virus was established to detect Chinese bee sacbrood virus.
基于中蜂囊状幼虫病病毒基因组序列AF469603中的RNA依赖的RNA聚合酶基因,建立该病毒的半套式RT-PCR检测方法。
3.
According to the nucleotide sequence of the U25631 gene of Salmonella registered in GeneBank, a set of primers were designed to amplify a specific 581 bp fragment by Semi-nested RT-PCR.
为建立一种快速、特异性和实用性强、灵敏度高的沙门氏菌检测方法,参考GeneBank中登录号为U25631的沙门氏菌基因序列,设计并合成引物,组成半套式RT-PCR,扩增片段为581bp。
5) Semi-nested PCR
半嵌套式RT-PCR
1.
The Study of WT1 Gene Expression in the Urine from Patients with Renal Diseases by Semi-nested PCR;
半嵌套式RT-PCR法检测肾病患者尿沉渣中WT1基因表达研究
6) clone and sequencing
RT-半嵌套式pcR
补充资料：嵌套式PCR
分子式：
CAS号：
性质：后一次PCR的引物互补于前一次PCR所扩增序列的内侧。
CAS号：
性质：后一次PCR的引物互补于前一次PCR所扩增序列的内侧。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条