Real-time quantitative PCR in determination of telomere length;

Detection of IgH rearrangements using real-time quantitative PCR and Its Reaction Parameters;

Principles of real-time quantitative PCR and its application in plant pathology;

287 Gy·min-1;total dose:7 Gy) exposuring,6 months later they were killed,then total RNA and total protein were extracted from thymic lymphomas and normal thymus tissue and cDNA was synthesized,the expressions of c-myc mRNA and protein in thymic lymphomas induced by ionizing radiation and normal thymus were detected by quantitative real-time PCR and Western blotting respectively.

The design principles and application in medicine ,molecular biology, food testing of the quantitative real-time PCR were reviewed in this paper.

Establishment of RT-PCR detecting relative expression of sBD-1-mRNA in Mongolian sheep

Method:IGF-IR mRNA expression in marrow cells from patients with MDS and normal controls was quantitatively determined by real time reverse transcript polymerase chain reaction(real time RT-PCR).

Real time PCR analysis showed that the expression levels of FSH1 and PQ-LRP genes were 44.

MATERIALS AND METHODS: After treated with 100 nmol/L MCLR for 24 h,the mRNA expression of protein phosphotase 2A subunits of human amniotic cells and human hepatocyte cells was measured by real time PCR.

In this study,real time PCR were used to monitor changed quantity of bacteria in rumens of 12 Tibetan sheep with seasonal shift,by collecting rumen content of grazing Tibetan sheep in alpine meadow in spring,summer,autumn and winter,respectively.