1) real-time transcription-polymerase chain reaction
实时定量-PCR
2) realtime PCR/RT-PCR
实时定量PCR/RT-PCR
3) real-time quantitative PCR
实时定量PCR
1.
Real-time quantitative PCR in determination of telomere length;
荧光实时定量PCR检测端粒长度
2.
Detection of IgH rearrangements using real-time quantitative PCR and Its Reaction Parameters;
IgH重排的实时定量PCR检测及反应参数研究
3.
Principles of real-time quantitative PCR and its application in plant pathology;
实时定量PCR的原理及其在植物病理学研究中的应用
4) Quantitative real-time PCR
实时定量PCR
1.
287 Gy·min-1;total dose:7 Gy) exposuring,6 months later they were killed,then total RNA and total protein were extracted from thymic lymphomas and normal thymus tissue and cDNA was synthesized,the expressions of c-myc mRNA and protein in thymic lymphomas induced by ionizing radiation and normal thymus were detected by quantitative real-time PCR and Western blotting respectively.
min-1,总剂量7Gy)照射BALB/c小鼠,建立小鼠胸腺淋巴瘤模型,6个月后处死小鼠,取辐射诱发小鼠胸腺淋巴瘤和正常胸腺,分别提取总RNA、合成cDNA和提取总蛋白,采用实时定量PCR方法和Western blotting方法分别检测辐射诱发胸腺淋巴瘤和正常胸腺组织c-myc基因mRNA和蛋白表达。
2.
The design principles and application in medicine ,molecular biology, food testing of the quantitative real-time PCR were reviewed in this paper.
笔者对实时定量PCR试验设计原则及其在医学、分子生物学、食品检测的应用上进行了概述。
5) RT-PCR
实时定量PCR
1.
Establishment of RT-PCR detecting relative expression of sBD-1-mRNA in Mongolian sheep
蒙古绵羊β-防御素基因相对表达水平实时定量PCR方法的建立
2.
Method:IGF-IR mRNA expression in marrow cells from patients with MDS and normal controls was quantitatively determined by real time reverse transcript polymerase chain reaction(real time RT-PCR).
方法:用实时定量PCR(real time RT-PCR)方法检测胰岛素样生长因子-I受体mRNA的表达量。
6) real time PCR
实时定量PCR
1.
Real time PCR analysis showed that the expression levels of FSH1 and PQ-LRP genes were 44.
方法应用斑点杂交和实时定量PCR技术筛选并检测差异基因;利用BLAST软件在GenBank数据库上进行同源序列比较。
2.
MATERIALS AND METHODS: After treated with 100 nmol/L MCLR for 24 h,the mRNA expression of protein phosphotase 2A subunits of human amniotic cells and human hepatocyte cells was measured by real time PCR.
材料与方法:FL和HL7702经100 nmol/L MCLR作用24 h后,采用实时定量PCR方法检测PP2A各亚基mRNA表达的变化。
3.
In this study,real time PCR were used to monitor changed quantity of bacteria in rumens of 12 Tibetan sheep with seasonal shift,by collecting rumen content of grazing Tibetan sheep in alpine meadow in spring,summer,autumn and winter,respectively.
采用瘤胃细菌通用引物和实时定量PCR技术,研究瘤胃细菌数量随季节的动态变化。
补充资料：immune PCR
分子式：
CAS号：
性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
CAS号：
性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条