荧光实时定量PCR,Real-time PCR,音标,读音,翻译,英文例句,英语词典

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1) Real-time PCR 点击朗读

荧光实时定量PCR

Detection of Streptococcus suis serotype 2 by Real-time PCR; 点击朗读

荧光实时定量PCR方法检测猪链球菌2型

Longissimus dorsal muscle was sampled to measure IMF content; and total RNA was extracted to determine H-FABP and PPARγ mRNA expression levels by real-time PCR.

选取不同日龄的雄性哈萨克羊和新疆细毛羊,屠宰后取背最长肌,用索氏抽提法检测肌内脂肪(Intamuscularfat,IMF)含量,用荧光实时定量PCR法检测心脏脂肪酸结合蛋白(heartfattyacid-bindingprotein,H-FABP)和过氧化物酶体增殖物激活受体γ(peroxisomeproliferator-activatedreceptorγ,PPARγ)基因表达的发育性变化极其对IMF含量的影响。

Methods Test 91 samples of confirmed and/or possible cases and 25 samples of healthy volunteers simultaneously by combining multiplex nested PCR with real-time PCR.

24%;荧光实时定量PCR检测阳性标本数68例,阳性检出率74。

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2) real-time quantitative PCR 点击朗读

荧光实时定量PCR

To analyze accurately the roles of growth hormone(GH),two growth hormone receptors(GHR1 and GHR2) and insulin-like growth factor-I(IGF-I) during early development of Nile tilapia Oreochromis niloticus,methods of real-time quantitative PCR to examine GH,GHR and IGF-I gene expression were established.

为了准确分析尼罗罗非鱼生长激素(growth hormone,GH)、生长激素受体(growth hormone receptors,GHRs)和胰岛素样生长因子I(Insulin-like growth factor-Ⅰ,IGF-Ⅰ)在早期发育阶段的作用,实验设计了尼罗罗非鱼GH、GHR、IGF-Ⅰ基因的特异性引物,提取垂体或肝脏总RNA并扩增出目的片段,将PCR产物克隆到pGEM-TEasy载体,经质粒PCR扩增、酶切和测序鉴定重组质粒,构建标准曲线等,成功建立了GH、GHR、IGF-Ⅰ基因荧光实时定量PCR检测方法。

Real-time quantitative PCR in determination of VEGF expression in endometrial carcinoma and peripheral blood and its clinical relevance;

方法:采用荧光实时定量PCR方法检测51例子宫内膜癌患者的癌组织和癌周组织、40例正常子宫内膜组织及其对应的患者外周血中的VEGF表达情况,分析其与临床病理参数之间的关系。

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3) real time PCR 点击朗读

荧光实时定量PCR

Methods: Using the technique of real time PCR, Western blotting and immunohistochemistry, the mRNA and protein expression of vimentin were measured in deciduas from 10 early onset severe preeclampsia patients (group A), 10 late onset severe preeclampsia patients (group B) and 10 normal women of full-term pregnancy (group C).

方法:采用荧光实时定量PCR(SYBR GREEN I assay)、Western blotting和免疫组织化学技术分别对晚发型重度子痫前期(A组)及早发型重度子痫前期(B组)和正常足月妊娠妇女(对照组,C组)各10例的波形蛋白mRNA及蛋白进行测定。

Real time PCR analysis showed that the positive expression of UGRP1mRNA in lung carcinoma was significantly lower than that in matching adjacent to normal lung tissues(P<0.

方法:采用免疫组织化学(SABC法)检测UGRP1在62例肺癌组织及其相对应的癌旁正常肺组织中的表达,应用荧光实时定量PCR检测20例新鲜肺癌组织和相对应的癌旁正常肺组织中UGRP1 mRNA的表达。

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4) qRT-PCR 点击朗读

荧光实时定量PCR

A Novel Method for the Determination of Recombinant Lentiviral Titer and Infectivity by qRT-PCR

应用荧光实时定量PCR方法检测重组慢病毒滴度及其感染效率

At the same time,we used qRT-PCR to verify eight differentially expressed genes in the SSH data,and to confirm the SSH technique is accurate and reproducible.

为了研究植物的抗旱机理,发掘抗旱基因,本研究以木本植物连香树为材料,通过抑制消减杂交(SSH)的方法,对连香树在干旱条件下的特异转录组进行研究,结合连香树在干旱条件下种子和二年生幼苗的生理生化变化,对连香树的抗旱机理进行了分析探讨;同时利用抑制消减文库中的差异表达基因片段,采用荧光实时定量PCR(qRT-PCR)方法,对8个差异表达基因片段的表达情况进行分析,验证SSH技术的可靠性。

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5) Real time quantitative PCR 点击朗读

荧光实时定量PCR

Methods In this study, immunohistochemistry and real time quantitative PCRwere used to detect survivin and hTERT expression in 30 cases of samples .

方法采用荧光实时定量PCR(Real time Quantitative PCR)方法和免疫组织化学S-P法分别在mRNA转录水平和蛋白水平检测30例胃癌组织及远癌切端正常组织中survivin和hTERT的表达。

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6) quantitative real time PCR 点击朗读

荧光实时定量PCR

Establishment and application of quantitative real time PCR assay for detecting duck viral enteritis virus;

鸭病毒性肠炎病毒荧光实时定量PCR检测方法的建立和应用

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补充资料：高压荧光灯用荧光粉

分子式：
CAS号：

性质：高压荧光灯中涂上Eu3+激活的钒酸钇或钒磷酸钇(YVO₄:Eu，Y(V，P)O₄:Eu)，可提高光效，改善显色性，增加了灯中的红色比和显色指数。YVO₄白色立方晶体耐温度猝灭，发光效率高，传递效率大，高压荧光汞灯主要用于街道，厂房和场地照明。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。

参考词条

实时荧光定量PCR(RT-FQ-PCR) 荧光实时定量PCR(Q-RT-PCR)

实时荧光定量PCR 荧光定量实时PCR 实时定量荧光PCR

说明：双击或选中下面任意单词，将显示该词的音标、读音、翻译等；选中中文或多个词，将显示翻译。
	您的位置：首页 -> 词典 -> 荧光实时定量PCR 1) Real-time PCR 荧光实时定量PCR 1. Detection of Streptococcus suis serotype 2 by Real-time PCR; 荧光实时定量PCR方法检测猪链球菌2型 2. Longissimus dorsal muscle was sampled to measure IMF content; and total RNA was extracted to determine H-FABP and PPARγ mRNA expression levels by real-time PCR. 选取不同日龄的雄性哈萨克羊和新疆细毛羊,屠宰后取背最长肌,用索氏抽提法检测肌内脂肪(Intamuscularfat,IMF)含量,用荧光实时定量PCR法检测心脏脂肪酸结合蛋白(heartfattyacid-bindingprotein,H-FABP)和过氧化物酶体增殖物激活受体γ(peroxisomeproliferator-activatedreceptorγ,PPARγ)基因表达的发育性变化极其对IMF含量的影响。 3. Methods Test 91 samples of confirmed and/or possible cases and 25 samples of healthy volunteers simultaneously by combining multiplex nested PCR with real-time PCR. 24%;荧光实时定量PCR检测阳性标本数68例,阳性检出率74。更多例句>> 2) real-time quantitative PCR 荧光实时定量PCR 1. To analyze accurately the roles of growth hormone(GH),two growth hormone receptors(GHR1 and GHR2) and insulin-like growth factor-I(IGF-I) during early development of Nile tilapia Oreochromis niloticus,methods of real-time quantitative PCR to examine GH,GHR and IGF-I gene expression were established. 为了准确分析尼罗罗非鱼生长激素(growth hormone,GH)、生长激素受体(growth hormone receptors,GHRs)和胰岛素样生长因子I(Insulin-like growth factor-Ⅰ,IGF-Ⅰ)在早期发育阶段的作用,实验设计了尼罗罗非鱼GH、GHR、IGF-Ⅰ基因的特异性引物,提取垂体或肝脏总RNA并扩增出目的片段,将PCR产物克隆到pGEM-TEasy载体,经质粒PCR扩增、酶切和测序鉴定重组质粒,构建标准曲线等,成功建立了GH、GHR、IGF-Ⅰ基因荧光实时定量PCR检测方法。 2. Real-time quantitative PCR in determination of VEGF expression in endometrial carcinoma and peripheral blood and its clinical relevance; 方法:采用荧光实时定量PCR方法检测51例子宫内膜癌患者的癌组织和癌周组织、40例正常子宫内膜组织及其对应的患者外周血中的VEGF表达情况,分析其与临床病理参数之间的关系。更多例句>> 3) real time PCR 荧光实时定量PCR 1. Methods: Using the technique of real time PCR, Western blotting and immunohistochemistry, the mRNA and protein expression of vimentin were measured in deciduas from 10 early onset severe preeclampsia patients (group A), 10 late onset severe preeclampsia patients (group B) and 10 normal women of full-term pregnancy (group C). 方法:采用荧光实时定量PCR(SYBR GREEN I assay)、Western blotting和免疫组织化学技术分别对晚发型重度子痫前期(A组)及早发型重度子痫前期(B组)和正常足月妊娠妇女(对照组,C组)各10例的波形蛋白mRNA及蛋白进行测定。 2. Real time PCR analysis showed that the positive expression of UGRP1mRNA in lung carcinoma was significantly lower than that in matching adjacent to normal lung tissues(P<0. 方法:采用免疫组织化学(SABC法)检测UGRP1在62例肺癌组织及其相对应的癌旁正常肺组织中的表达,应用荧光实时定量PCR检测20例新鲜肺癌组织和相对应的癌旁正常肺组织中UGRP1 mRNA的表达。更多例句>> 4) qRT-PCR 荧光实时定量PCR 1. A Novel Method for the Determination of Recombinant Lentiviral Titer and Infectivity by qRT-PCR 应用荧光实时定量PCR方法检测重组慢病毒滴度及其感染效率 2. At the same time,we used qRT-PCR to verify eight differentially expressed genes in the SSH data,and to confirm the SSH technique is accurate and reproducible. 为了研究植物的抗旱机理,发掘抗旱基因,本研究以木本植物连香树为材料,通过抑制消减杂交(SSH)的方法,对连香树在干旱条件下的特异转录组进行研究,结合连香树在干旱条件下种子和二年生幼苗的生理生化变化,对连香树的抗旱机理进行了分析探讨;同时利用抑制消减文库中的差异表达基因片段,采用荧光实时定量PCR(qRT-PCR)方法,对8个差异表达基因片段的表达情况进行分析,验证SSH技术的可靠性。更多例句>> 5) Real time quantitative PCR 荧光实时定量PCR 1. Methods In this study, immunohistochemistry and real time quantitative PCRwere used to detect survivin and hTERT expression in 30 cases of samples . 方法采用荧光实时定量PCR(Real time Quantitative PCR)方法和免疫组织化学S-P法分别在mRNA转录水平和蛋白水平检测30例胃癌组织及远癌切端正常组织中survivin和hTERT的表达。更多例句>> 6) quantitative real time PCR 荧光实时定量PCR 1. Establishment and application of quantitative real time PCR assay for detecting duck viral enteritis virus; 鸭病毒性肠炎病毒荧光实时定量PCR检测方法的建立和应用更多例句>> 补充资料：高压荧光灯用荧光粉分子式： CAS号：性质：高压荧光灯中涂上Eu3+激活的钒酸钇或钒磷酸钇(YVO₄:Eu，Y(V，P)O₄:Eu)，可提高光效，改善显色性，增加了灯中的红色比和显色指数。YVO₄白色立方晶体耐温度猝灭，发光效率高，传递效率大，高压荧光汞灯主要用于街道，厂房和场地照明。说明：补充资料仅用于学习参考，请勿用于其它任何用途。参考词条实时荧光定量PCR(RT-FQ-PCR) 荧光实时定量PCR(Q-RT-PCR)

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