2) nested PCR-RFLP
巢式PCR-RFLP
1.
he differentiation of Cryptosporidium parvum,C.andersoni,and C.meleagrides by Nested PCR-RFLP analysis of the SSU rRNA gene;
应用巢式PCR-RFLP方法鉴别微小隐孢子虫、安氏隐孢子虫和火鸡隐孢子虫的研究
2.
Isolation of two strains of Cryptosporidium from bovines by 18S rRNA gene nested PCR-RFLP
18Sr RNA基因巢式PCR-RFLP分析两株牛源隐孢子虫分离株
3) Semi-nested PCR
半巢式PCR
1.
Research on Promoter Region Methylation Status of Tumor Repressor Gene P16 in Human Hepatocellular Carcinoma by Using Methylation Sensitive Restriction Enzyme and Semi-nested PCR Assay;
限制性内切酶结合半巢式PCR法检测人肝癌P16抑癌基因启动子区甲基化研究
2.
A comparison of the detection sensitivity of five primer pairs(fOI1/rOI2c,fOI2/r23S1,fA2/rJ5,fP535/rP535,fP400/rP400)for citrus huanglongbing pathogen was carried out using simple PCR,semi-nested PCR and nested PCR.
半巢式PCR及巢式PCR的检测灵敏度远高于常规PCR,可检测出接近极限浓度10-7ng/μL,二者没有明显的差别。
3.
Semi-nested PCR method was established to detect trace ingredient of transgenic rapeseeds,which was 100 times more sensitive than the regular PCR.
建立了痕量转基因油菜籽成分的半巢式PCR(Semi-nested PCR)检测方法,灵敏度比常规PCR提高了100倍。
4) semi-nested polymerase chain reaction
半巢式PCR
1.
we detected for HPV16DNA in 152 cases of cervical carcinoma and 60 cases of normal cervical epithelia with semi-nested polymerase chain reaction.
采用半巢式PCR技术对152例维吾尔族妇女宫颈癌石蜡组织中HPV16DNA进行检测。
2.
To investigate the correlation between HPV16 infection and p53 protein expression in cervical carcinoma among Uigur women in Xinjiang,we detected for HPV16DNA and p53 protein in 80 cases of cervical carcinoma and 34 cases of normal cervical epithelia with semi-nested polymerase chain reaction and immunohistochemistry methods,respectively.
采用半巢式PCR法检测 80例新疆维吾尔族宫颈癌及 3 4例正常宫颈组织中HPV16DNA ,采用免疫组化法检测p5 3蛋白 ,分析其与宫颈癌发生的相关性 ,探讨HPV16感染和p5 3蛋白表达在新疆维吾尔族妇女宫颈癌发生中的作用。
5) semi-nested RT-PCR
半巢式RT-PCR
1.
Detection of respiratory syncytial virus by semi-nested RT-PCR;
半巢式RT-PCR法检测呼吸道合胞病毒
2.
Detection of Tobacco ringspot virus and Tomato ringspot virus by semi-nested RT-PCR;
烟草环斑病毒和番茄环斑病毒的半巢式RT-PCR检测
3.
Detection of Bean pod mottle virus by semi-nested RT-PCR in imported soybean;
半巢式RT-PCR检测进口大豆中菜豆荚斑驳病毒的研究
6) RT-seminested PCR
RT-半巢式PCR
1.
Reverse transcription polymerase chain reaction(RT-PCR),RT-seminested PCR and reverse transcription loop-mediated isothermal amplification(RT-LAMP) assays were compared to detect norovirus in oysters.
采用RT-PCR、RT-半巢式PCR(seminested PCR)和RT-环介导等温扩增(LAMP)三种分子生物学方法分别检测了牡蛎中经粪便污染的诺如病毒。
补充资料：RFLP
分子式：
CAS号：
性质：又称限制片长多态性。每一种基因型的DNA对某些限制性内切酶有固定的酶切位点，经酶作用后，在凝胶电泳图谱上产生固定的片段图谱。如基因型发生变化，则酶切位点或数目可能有差异，则产生的电泳图谱会有差异。这方法常用于遗传基因型的检查或病理检查。一般来讲高等生物个体都有特定的RFLP。
CAS号：
性质：又称限制片长多态性。每一种基因型的DNA对某些限制性内切酶有固定的酶切位点，经酶作用后，在凝胶电泳图谱上产生固定的片段图谱。如基因型发生变化，则酶切位点或数目可能有差异，则产生的电泳图谱会有差异。这方法常用于遗传基因型的检查或病理检查。一般来讲高等生物个体都有特定的RFLP。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条