1) cation ion-exchange chromatography
阳离子交换层析
1.
Methods SEC2 was purified by ultrafiltration,anion ion-exchange chromatography,and cation ion-exchange chromatography sequentially.
方法金葡液首先经超滤浓缩,之后再分别经阳离子交换层析和阴离子交换层析进行选择性分离SEC2,用SDS-PAGE电泳、HPLC法检测纯度及相对分子质量,飞行质谱测定精确分子质量,对其进行N-端氨基酸序列分析。
2) cation exchange liquid chromatography
阳离子交换层析法
3) ion-exchange chromatography
离子交换层析
1.
Formation of dimers in refolding of recombinant human interferon-γ by ion-exchange chromatography.;
离子交换层析复性重组人γ-干扰素折叠二聚体的形成
2.
Acetylcholinesterase(AChE) of Bombyx mori Linaeus,separated from the crude extract,was purified to electrophoretic homogeneity by Sephadex G-25 chromatography,DEAE-Sepharose Fast Flow ion-exchange chromatography and Sephacryl S-200 gel filtration,respectively.
采用了Sephadex G-25层析,DEAE-Sepharose Fast Flow离子交换层析和Sephacryl S-200凝胶过滤层析,对家蚕头部粗酶匀浆液中的乙酰胆碱酯酶进行纯化,得到的样品经聚丙烯酰胺凝胶电泳(PAGE)和SDS-PAGE检测均为一条带,用SDS-PAGE法测得其分子量为77。
3.
Then,BMP was isolated and purified by the methods of D301 ion-exchange chromatography(IEC)and Sephadex G-25 gel-filtration chromatography(GFC).
使用反相高效液相色谱(RP-HPLC)对重组酵母发酵液中一种牛肉风味肽(BMP)的稳定性进行了研究,并分别运用D301离子交换层析和Sephadex G-25凝胶过滤层析对其进行了分离纯化,最后利用RP-HPLC对目标肽进行分析检测。
4) DEAE iron-exchanged chromatography
DEAE离子交换层析
5) ion exchange chromatography
离子交换层析
1.
Study on the electrostatic interactions in cation exchange chromatography with molecular simulation;
基于分子模拟的离子交换层析中静电相互作用研究
2.
Crude inulinase preparation produced by Aspergillus ficuum SK004 was purified in a sequence of operations including decoloration by active carbon,ammonium sulfate fractionation,dialysis,ion exchange chromatography with DEAE—Sepharose CL—6B and column chromatography with Sephadex G—75.
通过活性炭脱色、硫酸铵分级沉淀、透析脱盐、DEAE-Sepharose CL-6B离子交换层析、Sephadex G-75凝胶色谱后,主要得到1种菊粉酶。
3.
To obtain high activity angiotensin converting enzyme inhibitor peptides (ACEIP), the method of ion exchange chromatography, gel filtration chromatography and RP-HPLC were used to separate the solution.
以玉米蛋白为原料酶解得到的酶解液具有降血压活性,为了得到高活性的降血压肽单体,本文采用了离子交换层析法、凝胶层析和RP-HPLC对酶解液进行了的分离纯化,得到了五个降血压多肽单体。
6) anion-exchange chromatography
阴离子交换层析
1.
To obtain the main peanut allergen Ara h2, defatted process, centrifugation, anion-exchange chromatography and membrane dialysis were used during the purification with the crude ground peanut as material.
结果显示,采用阴离子交换层析方法,制取的Arah2蛋白纯度达90%,得率为21。
2.
Alcaligenes faecalis penicillin G acylase(AfPGA) was isolated from recombinant Escherichia coli DH5α,and purified by a combination of DEAE-Sepharose anion-exchange chromatography and(Butyl)-Sepharose CL 4B hydrophobic chromatography.
采用DEAE-Sepharose阴离子交换层析和Butyl-Sepharose CL 4B疏水层析,从重组大肠杆菌DH5α菌体中分离纯化得到粪产碱杆菌青霉素G酰化酶(AfPGA)。
补充资料:阳离子
分子式:
CAS号:
性质:电解时向阴极迁移的带正电荷的粒子,也称阳离子,如Na+,NH4+,Al3+等。
CAS号:
性质:电解时向阴极迁移的带正电荷的粒子,也称阳离子,如Na+,NH4+,Al3+等。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条