Then SIgA was purified twice by Macro-prep High Q ion-exchange chromatography,and 22 mg/mL SIgA had been obtained.

After EGF-IL-18 inclusion body was repeatedly washed by 2 mol/L urea and 1%Triton X-100,the denatured EGF-IL-18 wasrefolded by ion-exchange chromatography using the urea linear gradient strategy.

Formation of dimers in refolding of recombinant human interferon-γ by ion-exchange chromatography.;

Acetylcholinesterase(AChE) of Bombyx mori Linaeus,separated from the crude extract,was purified to electrophoretic homogeneity by Sephadex G-25 chromatography,DEAE-Sepharose Fast Flow ion-exchange chromatography and Sephacryl S-200 gel filtration,respectively.

Then,BMP was isolated and purified by the methods of D301 ion-exchange chromatography(IEC)and Sephadex G-25 gel-filtration chromatography(GFC).

Study on the electrostatic interactions in cation exchange chromatography with molecular simulation;

Crude inulinase preparation produced by Aspergillus ficuum SK004 was purified in a sequence of operations including decoloration by active carbon,ammonium sulfate fractionation,dialysis,ion exchange chromatography with DEAE—Sepharose CL—6B and column chromatography with Sephadex G—75.

To obtain high activity angiotensin converting enzyme inhibitor peptides (ACEIP), the method of ion exchange chromatography, gel filtration chromatography and RP-HPLC were used to separate the solution.

To obtain the main peanut allergen Ara h2, defatted process, centrifugation, anion-exchange chromatography and membrane dialysis were used during the purification with the crude ground peanut as material.

Alcaligenes faecalis penicillin G acylase(AfPGA) was isolated from recombinant Escherichia coli DH5α,and purified by a combination of DEAE-Sepharose anion-exchange chromatography and(Butyl)-Sepharose CL 4B hydrophobic chromatography.