2) universal primer
通用引物
1.
Detecting enterovirus by reverse transcription nested-polymerase chain reaction established with universal primers;
应用通用引物建立巢式逆转录聚合酶链反应检测肠道病毒(英文)
2.
A rapid quantitative polymerase chain reaction(QPCR) analysis method with universal primers was developed to detect cell densities of the enteric pathogenic bacteria from 5 surface water of Xi'an City for 4 months continuously.
采用通用引物,通过实时荧光定量PCR方法(QPCR),对西安市5处地表水体中肠道病原细菌的细胞密度进行4个月的连续检测,并将QPCR检测结果与滤膜法测得的大肠菌群CFU值进行比较分析。
3.
In order to investigate the application values of polymerase chain reaction(PCR) technique in detection of pathogenic bacteria in surface water,the universal primers were designed and synthesized according to the high conversation of 16S rRNA gene.
为了探索通用引物PCR方法在地表水病原细菌检测中的应用价值,利用16S rRNA基因的高度保守性,设计并合成细菌的通用引物,采用合成的引物扩增参考菌株及西安市区不同地表水样,并对PCR产物分别进行序列测定及序列同源性分析,同时检测水样中的细菌总数和粪大肠杆菌浓度。
3) universal primers
通用引物
1.
Development, optimization and application of the expression analysis platform based on multiplex quantitative RT-PCR using fluorescent universal primers
基于荧光通用引物的多重定量RT-PCR基因表达谱分析平台的构建、优化及其应用
2.
The detection method for various enteroviruses in water sample by RTPCR with universal primers was established.
建立了一种利用通用引物RT-PCR技术检测水中肠道病毒的方法。
3.
One was sent for blood culture (BC), the other was detected by way of 16SrRNA gene Polymerase Chain Reaction(PCR) with universal primers of bacterial and PCR products were analyzed by electrophoresis using agarose ge.
将每份标本一分为二分别行血培养和用细菌通用引物行16SrRNA基因聚合酶链反应检测,用琼脂糖凝胶电泳分析PCR产物,比较两种方法在检测细菌病原体时的快速性和敏感性,分析血培养及药敏结果。
4) PCR primer
PCR引物
1.
Combining the author s working experiences, we introduce here several methodologies on improving the PCR products, such as the disciplines which we should abide by when we design PCR primers; how to determine the appropriate annealing temperature of the PCR reaction; how to improve the amplifying efficiency of the GC rich region.
结合作者的工作实际 ,着重介绍了有效提高PCR产物的几种方法 :如怎样高效快速地设计PCR引物 ,怎样尽快确定PCR反应的最适退火温度 ,如何提高GC富集区的扩增效率等 ,为分子生物学工作者提供一些可以借鉴的方法及经验。
5) huge primer PCR
巨引物PCR
1.
By using huge primer PCR Cys86 (TGC) of PoIFN-α was mutated to Tyr(TAC), and the first code TGT was simultaneously changed to TGC, which is a bias code of E.
用巨引物PCR法介导的定点突变把猪α干扰素 (PoIFN α)第 86位Cys(TGC)突变为Tyr(TAC) ,同时将其成熟蛋白N端第一个密码子TGT同义突变大肠杆菌偏爱的密码子TGC ,构建了大肠杆菌融合基因表达载体pGEX IFN ,表达产物占菌体总蛋白的 2 0 %。
补充资料:通用
1.普遍使用。 2.某些写法不同而读音相同或意义相通的汉字彼此可以换用。如:"糕"与"餻","由"与"繇"。
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