This article’s purpose is via measure the quantity of the collagen in pig lung to find the new approach to all-around utilize the pig lung.

The fresh hog lung was homogenated and centrifugated with 35%～55% saturation ammonium sulfate fraction.

To evaluate the difference of position and morphometry of pulmonary artery and vein of isolated pig lung , through making cast specimens .

This trial tried to compare the result of simultaneously vaccinating pigs with foot and mouth disease vaccine(the FMD vaccine) and the double mixed vaccine for classic swine fever and Pasteurellosis suum(the mixed CSF and PS vaccine),with the result of respectively vaccinating pigs with the FMD vaccine and the mixed CSF and PS vaccine during a month.

Based on a comparative study of two coating methods, with mycoplasm hyopneumoniae (antigen) or its antibody, a highly sensitive and accurate enzyme linked immunosorbent assay (ELISA) method has been developed for direct determination of biotin in the transformed yeast cultural media.

Method: Mycoplasm hyopneumoniae was used to coat the microtiter plate and its antibody in rabbit and goat anti-rabbit IgG incorporated biotin were linked to the plate successively to form a fixed phase of biotin which, after optimization of the experimental conditions, was applied in the followed competition inhibition reaction to determine bio.