Study on technology of ergosterol production by sugarcane molasses

A high-biomass,iron-enriched yeast strain R4-5 was obtained after gradual screening and fermenting through 25 yeast strain, treated it with 60Co mutation ,then got a positive mutation yeast strain R4-5-27 that contain more biomass and iron,and this yeast strain can used sugarcane molasses as the culture medium.

In order to reduce the produce cost of Bacillus subtilis,and find a new way to comprehensively utilize industrial waste water,Sugarcane molasses and waste water of MGS were used as main material of the liquid fermentation medium to culture Bacillus subtilis.

Using sugar-cane molasses to produce iron-enriched yeast and analyzing the percentage of organic iron of the yeast cell;

Study on the condition of feed yeast fermentation with sugar-cane molasses;

The iron-enriched yeast was produced by using sugar-cane molasses,after single factor and orlhogonal design experiments,the optimal combinations were as follow:the initial pH of culture medium is 5.