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1)  preprochymosin
凝乳酶原前体
1.
Then based on blast the sequence of Ovis aries preprochymosin cDNA (Accession No: X53037) reported in GeneBank, two pairs of primers were designed to clone Xinong Saanen Goat preprochymosin with the 5 d postparturition abomasum cDNA as template by RT-PCR method.
本研究参照GenBank收录的绵羊凝乳酶原前体cDNA序列(GenBank No:X53037)设计RT-PCR、PCR与巢式引物,以新生5天的西农萨能羊公羔羊为研究对象,利用手术法采集羔羊皱胃组织,利用Trizol法提取皱胃组织总RNA,经过电泳鉴定后,以总RNA为模板利用RT-PCR方法获得西农萨能羊凝乳酶原前体cDNA序列,以cDNA序列为模板进行扩增和T载体连接测序。
2)  prerennin
前凝乳酶
3)  prochymosin [prəu'kaiməsin]
凝乳酶原
1.
Cloning and phylogenetic analysis of bovine prochymosin gene;
牛凝乳酶原基因克隆及序列的进化分析
2.
The Kpn I-Kpn I fragment of prochymosin gene was inserted into the phagemidvector:pBluescript.
以phagemid,pBluescript为载体,把牛凝乳酶原基因的KpnI-KpnI片段插入,将牛凝乳酶45位Cys用Ala取代,根据遗传密码子的兼并性,在其附近引入限制性内切酶ClaI位点。
3.
During the work of site\|directed mutagenesis at disulfide bond Cys206\|Cys210 of prochymosin,it was found that the corresponding template sequence had the potential to form a loop\|stem structure with free energy of -16 1 kcal/mol,which prevent the template from pairing with primer and,in turn,the synthesis of the mutated DNA strand.
在对凝乳酶原二硫键Cys2 0 6 Cys2 10进行定位突变过程中发现 ,在相应的模板序列中有自身形成自由能为 - 16 1kcal/mol的茎环结构倾向 ,妨碍与引物结合 ,从而难以合成突变的DNA ,采用快退火可解决此矛盾。
4)  PIVKA-Ⅱ
凝血酶原前体蛋白
1.
【Objective】 To investigate the influence of the third generation cephalosporin on vitamin K nutrition status in children by determining the concentration of proteins induced by vitamin K absence or antagonism(PIVKA-Ⅱ) in plasma;Preventive treatment with vitamin K1 to the children treated with antibiotics to reverse the status of vitamin K deficiency.
【目的】通过血浆凝血酶原前体蛋白(PIVKA-Ⅱ)的测定,探讨第三代头孢菌素对儿童维生素K营养状况的影响。
5)  Des-carboxylated
血清凝血酶原前体蛋白
1.
【Objective】 To study the status of vitamin K in(LBKDB) infants and their mothers by Des-carboxylated Prothrombin(PIVKA-Ⅱ).
【目的】以血清凝血酶原前体蛋白(PIVK-Ⅱ)来研究晚发型维生素K缺乏性出血(late vitamin K deficiencybleeding,LVKDB)患儿及其母亲维生素K营养状况,观察不同剂量维生素K对PIVKA-Ⅱ的影响。
6)  PIVKA-II
凝血酶原前体蛋白(PIVKA-Ⅱ)
补充资料:胰凝乳蛋白酶原

胰凝乳蛋白酶原 chymotrypsinogen

胰凝乳蛋白酶原 chymotrypsinogen 为胰凝乳蛋白酶的前体物质。牛的胰凝乳蛋白酶原a氨基酸残基245个,分子量24500。

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