Expression of functional formylpeptide receptor FPR by primary human malignant glioma cells;

Objective To observe the effects of Nordy,a synthetic compound,on the expression of formylpeptide receptor(FPR)and vascular endothelial growth factor(VEGF)in orthotopically implanted tumor in the brain of human malignant glioma-bearing nude mice and explore the mechanism of anti-tumor effect of Nordy.

To investigate the effects of nordy(25-100 μmol·L~(-1)) on the function of formylpeptide receptor(FPR) of malignant human glioma cells,human glioblastoma cell line U87 was used to detect its proliferation,migration,calcium mobilization,vascular endothelial growth factor(VEGF) mRNA and protein levels after activation of FPR by its agonist N-formyl-methionyl-leucyl-phenylalanine(fMLF).

Co -immuno-precipitate method was used to confirm human formyl peptide receptor-like1(FPRL1)binding specifically with DR4CD(the cytoplsmic domain of DR4)in HEK293T cells.

In this thesis, an FPRL1-based high-throughput screening (HTS)assay was developed and applied to a large-scale compound screening campaign.

Meanwhile, a series of preliminary studies were conducted on the orphanG-protein coupled receptor FPRL2, another member of the FPR family.