1) SYBR real-time fluorescent quantitative polymerase chain reaction
SYBR实时荧光定量聚合酶链反应
2) Power SYBR Green real time RT-PCR
Power SYBR Green实时荧光反转录聚合酶链式反应
3) real-time reverse transcription polymerase chain reaction
实时荧光定量反转录聚合酶链反应
1.
Methods The expression of PBMC IP-10 mRNA in 42 children with KD and 40 healthy children were assayed by real-time reverse transcription polymerase chain reaction(real-time RT-PCR).
方法实时荧光定量反转录聚合酶链反应(real-time RT-PCR)检测42例KD及40例健康儿童PBMC中IP-10 mRNA表达。
4) real-time fluorescence reverse transcription polymerase chain reaction (RT-PC
荧光实时定量反转录-聚合酶链反应
5) real-time polymerase chain reaction
实时荧光定量聚合酶链反应
1.
Methods DNASE1 mRNA expression was detected by real-time polymerase chain reaction and its alternatively spliced transcripts were performed by capillary electrophoresis.
方法 以实时荧光定量聚合酶链反应 (real- time PCR)方法检测 DNASE1的 m RNA表达水平 ,以毛细管电泳技术分析 m RNA编码区替代剪接体 ,结合单核苷酸多态性 (single nucleotide polymorphisms,SNPs)单倍型了解基因结构对表达的影响。
6) real-time fluorescence relative quantitative RT-PCR
实时荧光定量-逆转录-聚合酶链反应
补充资料：聚合酶链反应
聚合酶链反应
polymerase chain reaction,PCR
是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA）片断大量增扩，达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体，因其假阳性率较高，故应谨慎使用。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条