1) Real-time RT-PCR
实时荧光定量逆转录聚合酶链反应
1.
MethodsPart I:Real-time reverse transcription polymerase chain reaction (Real-time RT-PCR)was used to investigate HOXA5mRNA expression in 60 cases of primary breast cancer and 24 cases of benign breast lesion.
方法 第一部分 1、采用实时荧光定量逆转录聚合酶链反应(real-time reverse transcription polymerase chain reaction，Real-time RT-PCR)技术，检测60例新鲜乳腺癌组织及24例良性乳腺病变中HOXA5mRNA表达。
2) Real-time fluorescent quantitative reverse transcriptase polymerase chain reaction
实时荧光定量逆转录聚合酶链反应
1.
METHODS: Real-time fluorescent quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect the level of hPMS2 mRNA respectively in 41 cases of gastric cancer and 37 cancer-adjacent mucosa samples of atrophic gastritis and 25 cases of chronic atrophic gastritis and 20 cases of chronic superf icial gas-tritis.
方法:应用实时荧光定量逆转录聚合酶链反应(RT-PCR)技术对41例胃癌患者的癌组织、37例癌旁萎缩性胃炎组织、25例慢性萎缩性胃炎组织及20例慢性浅表性胃炎组织中hPMS2 mRNA进行定量检测,以三磷酸甘油醛脱氢酶基因(hGAPDH)为内参照。
3) real time quantitative RT-PCR
实时荧光定量逆转录聚合酶链反应
1.
Methods Adult Wistar rats were exposed to hypobaric hypoxic environment for 2 hours following which the expression of AngⅡwere observed at 3 hours,24 hours,3 days,7 days and 14 days with real time quantitative RT-PCR and Western-blotting,respectively.
L-1O2)2h后,分别在3h、24h、3d、7d和14d,通过实时荧光定量逆转录聚合酶链反应、蛋白免疫印迹法检测AngⅡ在视网膜中的表达情况。
4) real-time fluorescence relative quantitative RT-PCR
实时荧光定量-逆转录-聚合酶链反应
5) real-time quantitative reverse transcription polymerase chain reaction
实时荧光定量逆转录-聚合酶链反应
1.
The COL3A1 mRNA was measured by real-time quantitative reverse transcription polymerase chain reaction(RT-PCR).
方法应用基因芯片技术和实时荧光定量逆转录-聚合酶链反应(RT-PCR)技术,检测Ⅲ型胶原α链在不同舌苔中的表达水平。
6) Real-time RTpolymerase chain reaction
实时荧光定量逆转录聚合酶链式反应
补充资料：聚合酶链反应
聚合酶链反应
polymerase chain reaction,PCR
是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA）片断大量增扩，达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体，因其假阳性率较高，故应谨慎使用。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条