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1)  TaqMan-based Real-time PCR
TaqMan探针实时荧光定量聚合酶链反应
2)  TaqMan probe real-time quantitative polymerase chain reaction
TaqMan探针实时定量聚合酶链反应
1.
Objective Detection of cell-free fetal DNA in maternal serum by TaqMan probe real-time quantitative polymerase chain reaction(real-time quantitative PCR) and on the basis of it s amount to discuss the feasibility as a new prenatal screening indicator of Down s syndrome.
目的应用TaqMan探针实时定量聚合酶链反应(Real-time quantitative PCR)技术检测孕妇血清中胎儿游离DNA含量,探讨以其含量变化作为Down’s综合征产前筛查新指标的可行性。
3)  Real-time TaqMan PCR
实时荧光TaqMan聚合酶链反应
4)  real-time PCR with Taqman probe
Taqman荧光探针实时定量PCR
5)  FQ-PCR (Fluoresce Quantity-Polymerase chain reaction)
FQ-PCR(荧光探针定量聚合酶链反应)
6)  real-time reverse transcription polymerase chain reaction
实时荧光定量反转录聚合酶链反应
1.
Methods The expression of PBMC IP-10 mRNA in 42 children with KD and 40 healthy children were assayed by real-time reverse transcription polymerase chain reaction(real-time RT-PCR).
方法实时荧光定量反转录聚合酶链反应(real-time RT-PCR)检测42例KD及40例健康儿童PBMC中IP-10 mRNA表达。
补充资料:聚合酶链反应


聚合酶链反应
polymerase chain reaction,PCR

是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA)片断大量增扩,达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体,因其假阳性率较高,故应谨慎使用。
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