1) cultural DNA
文化DNA
2) DNA library
DNA文库
1.
Construction of genomic DNA library of the mirror carp;
黑龙江镜鲤基因组DNA文库的构建
2.
The genomic DNA library of Paenibacillus sp.
提取该菌株的染色体DNA,以pUC18(lac-)为载体,构建其DNA文库;在含有X-gal的LB平板上筛选该文库,得到6个蓝色菌落;对阳性克隆中插入的DNA片段序列测定,鉴定出1个编码全长为2 028 bp并携带有组成型启动子的β-半乳糖苷酶基因。
3.
Bacterium Proteus vulgaris TWN3 strain was isolated from tissue of dead southern sheatfish Silurus meridionalis in which the DNA library was constructed successfully.
普通变形杆菌TWN3分离自死亡大口鲇,菌的DNA文库已构建成功,通过免疫印迹法从文库中筛选到TWN3抗原片段PV4,将其克隆到真核表达载体pcDNA3上,构建DNA疫苗pcDNA3-PV4。
3) DNA methylation
DNA甲基化
1.
DNA methylation and autoimmune diseases;
DNA甲基化与自身免疫性疾病
2.
Microarray-based methods to identify DNA methylation in cancer;
芯片技术与肿瘤中DNA甲基化研究
3.
Relationship of nutrition with cancer and its epigenetic DNA methylation mechanism;
营养与肿瘤表观遗传学关系的研究进展——DNA甲基化机制
4) DNA Purification
DNA纯化
1.
Sequence based typing (SBT) becomes the best method of HLA typing because of development of DNA amplification, DNA purification and other technology from 1 990 s, SBT is gold standard of HLA gene typing on organ transplantation, human genetics and disease association.
20世纪90年代以来DNA扩增、DNA纯化和人类基因成果对HLA领域的渗透,使得碱基序列测序方法(sequencebasedtyping,SBT)成为一种可直接阅读HLA系统复杂性的基因物质的最佳技术,并在器官移植、人类遗传学、疾病关联等研究方面被认为是HLA基因分析的金标准。
2.
DNA extraction includes two steps of cell lysis and DNA purification.
DNA提取分细胞裂解和DNA纯化2步,对细胞裂解比较了珠磨匀浆法、反复冻融法、十二烷基磺酸钠(sodium dodecyl sul-fate,SDS)裂解法等7种方法;对DNA纯化比较了酚/氯仿纯化法和胶回收纯化法。
5) total DNA transformation
全DNA转化
1.
In ion beammediated total DNA transformation, the total DNA consists of all of genetic information in donor, and have multilateral effects on recipient, so there was no common rate of transformation to measure its effect, which means that it was very difficult to decide an optimum condition of transformation such as influence.
在离子束介导外源全DNA转化中,外源DNA包含有供体的所有遗传信息,对受体的影响是多方面的,因此转化效果无法用统一的转化率来衡量,这也增加了转化过程中最佳转化条件的选择难度。
6) DNA Fragmentation
DNA片断化
1.
Methods In this study,a human normal liver cell line L-02 was treated with different concentrations of MCLR for toxicological investigation such as morphological changes, DNA fragmentation and mitochrondria membrane potential.
方法以不同浓度的微囊藻毒素LR(MCLR)处理L-02肝细胞,通过光镜和电镜下的形态观察、DNA片断化分析、线粒体膜电位变化等了解MCLR对肝细胞的毒性效应。
补充资料：DNA分子花窗(沿B―DNA轴线方向看)
[图]
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条