Immune efficacy of bivalent combinations and double fusion DNA vaccine from Mycobacterium bovis

Objective To compare the effect of macrophage-derived chemokine(MDC)and Shiga toxin B subunit(STxB)gene on the neutralizing antibodies and specific CTL response induced by Coxasckievirus B3(CVB3)VP1 fusion DNA vaccine,as wells as the protective effect of the genes on the mice challenged with CVB3.

DNA methylation and autoimmune diseases;

Microarray-based methods to identify DNA methylation in cancer;

Relationship of nutrition with cancer and its epigenetic DNA methylation mechanism;

Sequence based typing (SBT) becomes the best method of HLA typing because of development of DNA amplification, DNA purification and other technology from 1 990 s, SBT is gold standard of HLA gene typing on organ transplantation, human genetics and disease association.

DNA extraction includes two steps of cell lysis and DNA purification.

In ion beammediated total DNA transformation, the total DNA consists of all of genetic information in donor, and have multilateral effects on recipient, so there was no common rate of transformation to measure its effect, which means that it was very difficult to decide an optimum condition of transformation such as influence.