细胞内RT-PCR,in-cell reverse transcription poly-merase chain reaction(in-cellRT-PCR),音标,读音,翻译,英文例句,英语词典

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1) in-cell reverse transcription poly-merase chain reaction(in-cellRT-PCR) 点击朗读

细胞内RT-PCR

2) single-cell RT-PCR 点击朗读

单细胞RT-PCR

Methods: Human neutrophils were isolated and purified from volunteers,total RNA was extracted and a regular RT-PCR aiming at IL-8Rβ mRNA was performed to ascertain its expression profile in human neutrophils and optimize the reaction conditions for the following single-cell RT-PCR procedures.

方法:采用倍比稀释相对定量单细胞RT-PCR方法分离纯化人外周血中性粒细胞并提取总RNA,针对IL-8RβmRNA设计引物,进行RT-PCR确认IL-8Rβ在中性粒细胞中的基因表达并优化反应条件。

3) Single cell nested RT-PCR 点击朗读

单细胞巢式RT-PCR

4) in cell PCR 点击朗读

细胞内PCR

例句>>

5) reverse transcription-polymerase chain reaction 点击朗读

RT-PCR

With beta-actin gene as the internal standard,reverse transcription-polymerase chain reaction(RT-PCR) was used to examine the expression of fms-like tyrosine kinase(Flt-1) mRNA in endometrium of Small Tail Han sheep and Northeast Fine-wool sheep.

以β-actin基因作为内源性内标,采用RT-PCR方法检测了Flt-1基因mRNA在东北细毛羊、小尾寒羊妊娠期不同阶段子宫内膜中的表达量。

MethodsThe expression of FEZ1 mRNA in 50 cases of esophageal carcinoma and 50 cases of normal esophageal mucoma were detected by reverse transcription-polymerase chain reaction(RT-PCR).

方法采用RT-PCR法检测50例食管鳞癌及50例正常食管组织中FEZ1 mRNA的表达情况,分析其与食管鳞癌患者各临床病理指标的关系。

Methods The mRNA of Ki-67 and VEGF were detected using reverse transcription-polymerase chain reaction(RT-PCR) in 55 RCC tissues and 20 normal renal tissues.

方法应用半定量RT-PCR技术检测55例肾癌组织和20例正常肾组织Ki-67和VEGFmRNA表达情况。

更多例句>>

6) Reverse transcription polymerase chain reaction 点击朗读

RT-PCR

Then the level of VEGF mRNA in kidney was determined by reverse transcription polymerase chain reaction (RT-PCR); the expression of VEGF and VEGF.

【方法】取3月龄、12月龄大鼠作为对照组,24月龄大鼠作为观察组各6只,提取其肾脏组织的RNA,应用逆转录聚合酶链反应技术(RT-PCR)检测肾脏组织内VEGFmRNA转录水平,以及应用免疫组化技术检测肾小球VEGF和VEGFR蛋白的表达;应用酶联免疫法检测肾组织培养液的VEGF总含量。

Methods Reverse transcription polymerase chain reaction (RT PCR) was used to evaluate the amount of insulin like growth factor mRNA in exercise induced damaged skeletal muscle, and specimens were used for ultrastructural observation.

方法利用IGF引物对不同损伤程度骨骼肌标本进行RT-PCR检测,同时观查损伤骨骼肌超微结构改变。

Methods NIT-1 cells were exposed to cyclosporin A (10 μmol/L) for 24 and 48 h respectively, after which the amount of insulin release was determined by means of radioimmunoassay (RIA), and the differential expressions of Nuox23, Cox7c and Atp5K genes assessed by semi-quantitative reverse transcription polymerase chain reaction.

放射免疫测定法(RIA)检测胰岛素释放,半定量RT-PCR检测CsA处理NIT-1胰岛β细胞后线粒体氧化磷酸化酶系中的几种主要成员(Nuox23、Cox7c和Atp5K)在mRNA水平上的表达。

更多例句>>

补充资料：精液内细胞个别不同类型细胞检查

精液内细胞个别不同类型细胞检查

诊法。射精液的个别细胞形态的分类测定，是精液分析要取得的参数之一。取已用瑞氏染色法或吉姆染色法染好的精液涂片，在光镜下计数100个头部卵圆，中段形子、尾子蜷曲的正常精子，同时在一个视野内，计数欲测的不成熟精子或其他形态的细胞。按下列公式计算：C=〓〓N×S〓〓100〓〓(式中：C——欲测得的细胞；N—— 在计数 100个精子时同一视野下的细胞数；S——1ml精液中的精子数)。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。

参考词条

实时RT-PCR RT-PCR法原位RT-PCR 多重RT-PCR 巢式RT-PCR

RTPCR 全细胞PCR 单细胞PCR 全细胞PCR法

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	您的位置：首页 -> 词典 -> 细胞内RT-PCR 1) in-cell reverse transcription poly-merase chain reaction(in-cellRT-PCR) 细胞内RT-PCR 例句>> 2) single-cell RT-PCR 单细胞RT-PCR 1. Methods: Human neutrophils were isolated and purified from volunteers,total RNA was extracted and a regular RT-PCR aiming at IL-8Rβ mRNA was performed to ascertain its expression profile in human neutrophils and optimize the reaction conditions for the following single-cell RT-PCR procedures. 方法:采用倍比稀释相对定量单细胞RT-PCR方法分离纯化人外周血中性粒细胞并提取总RNA,针对IL-8RβmRNA设计引物,进行RT-PCR确认IL-8Rβ在中性粒细胞中的基因表达并优化反应条件。 3) Single cell nested RT-PCR 单细胞巢式RT-PCR 4) in cell PCR 细胞内PCR 例句>> 5) reverse transcription-polymerase chain reaction RT-PCR 1. With beta-actin gene as the internal standard,reverse transcription-polymerase chain reaction(RT-PCR) was used to examine the expression of fms-like tyrosine kinase(Flt-1) mRNA in endometrium of Small Tail Han sheep and Northeast Fine-wool sheep. 以β-actin基因作为内源性内标,采用RT-PCR方法检测了Flt-1基因mRNA在东北细毛羊、小尾寒羊妊娠期不同阶段子宫内膜中的表达量。 2. MethodsThe expression of FEZ1 mRNA in 50 cases of esophageal carcinoma and 50 cases of normal esophageal mucoma were detected by reverse transcription-polymerase chain reaction(RT-PCR). 方法采用RT-PCR法检测50例食管鳞癌及50例正常食管组织中FEZ1 mRNA的表达情况,分析其与食管鳞癌患者各临床病理指标的关系。 3. Methods The mRNA of Ki-67 and VEGF were detected using reverse transcription-polymerase chain reaction(RT-PCR) in 55 RCC tissues and 20 normal renal tissues. 方法应用半定量RT-PCR技术检测55例肾癌组织和20例正常肾组织Ki-67和VEGFmRNA表达情况。更多例句>> 6) Reverse transcription polymerase chain reaction RT-PCR 1. Then the level of VEGF mRNA in kidney was determined by reverse transcription polymerase chain reaction (RT-PCR); the expression of VEGF and VEGF. 【方法】取3月龄、12月龄大鼠作为对照组,24月龄大鼠作为观察组各6只,提取其肾脏组织的RNA,应用逆转录聚合酶链反应技术(RT-PCR)检测肾脏组织内VEGFmRNA转录水平,以及应用免疫组化技术检测肾小球VEGF和VEGFR蛋白的表达;应用酶联免疫法检测肾组织培养液的VEGF总含量。 2. Methods Reverse transcription polymerase chain reaction (RT PCR) was used to evaluate the amount of insulin like growth factor mRNA in exercise induced damaged skeletal muscle, and specimens were used for ultrastructural observation. 方法利用IGF引物对不同损伤程度骨骼肌标本进行RT-PCR检测,同时观查损伤骨骼肌超微结构改变。 3. Methods NIT-1 cells were exposed to cyclosporin A (10 μmol/L) for 24 and 48 h respectively, after which the amount of insulin release was determined by means of radioimmunoassay (RIA), and the differential expressions of Nuox23, Cox7c and Atp5K genes assessed by semi-quantitative reverse transcription polymerase chain reaction. 放射免疫测定法(RIA)检测胰岛素释放,半定量RT-PCR检测CsA处理NIT-1胰岛β细胞后线粒体氧化磷酸化酶系中的几种主要成员(Nuox23、Cox7c和Atp5K)在mRNA水平上的表达。更多例句>> 补充资料：精液内细胞个别不同类型细胞检查精液内细胞个别不同类型细胞检查诊法。射精液的个别细胞形态的分类测定，是精液分析要取得的参数之一。取已用瑞氏染色法或吉姆染色法染好的精液涂片，在光镜下计数100个头部卵圆，中段形子、尾子蜷曲的正常精子，同时在一个视野内，计数欲测的不成熟精子或其他形态的细胞。按下列公式计算：C=〓〓N×S〓〓100〓〓(式中：C——欲测得的细胞；N—— 在计数 100个精子时同一视野下的细胞数；S——1ml精液中的精子数)。 说明：补充资料仅用于学习参考，请勿用于其它任何用途。参考词条实时RT-PCR RT-PCR法原位RT-PCR 多重RT-PCR 巢式RT-PCR

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