1) Western dot assay
Western斑点印迹分析
2) Western blot
Western印迹分析
1.
HSP72 mRNA and HSP70 expression in heart and vessel tissues of both simulated weightless and control rats exposed to heat stress (ambient temperature, Ta=43℃) and recovered at Ta of 25℃ for 1 h (CON H 1, SUS H 1) or 2 h (CON H 2, SUS H 2) were analyzed using Northern blot and Western blot.
用Northern杂交与Western印迹分析检测 4周模拟失重大鼠热应激后并在室温下恢复 1h (SUS H1)或 2h (SUS H2 )心肌、血管组织HSP70表达的变化。
3) Western blotting
Western印迹分析
1.
The protein expression of AGT and AT 1 in basilar, carotid, femoral, and mesenteric arterial tissues were demonstrated by Western blotting and normalized by β actin for quantitat.
用Western印迹分析比较 4周模拟失重 (SUS)组与同步对照 (CON)组大鼠基底动脉、颈总动脉、股动脉和肠系膜动脉组织AGT及AT1 的蛋白表达变化。
4) Western blot analysis
Western印迹分析
1.
Western blot analysis showed the exogenous OsGSTL1 was expressed in transformed yeast.
Western印迹分析表明外源OsGSTL1基因在转基因酵母中表达,分析半乳糖诱导表达的酵母粗提液的谷胱甘肽转移酶活性表明:转基因酵母的谷胱甘肽转移酶较非转基因酵母和未诱导的酵母高,说明OsGSTL1在转基因酵母中受半乳糖的诱导表达,具有谷胱甘肽转移酶活性。
5) Western-blot
Western斑点印迹法
1.
The HSP27/60/70 expression level in right atrial samples was determined by Western-blot,while the expression in serum samples was determined by enzyme-linked immunosorb.
方法选取瓣膜置换手术患者40例,其中20例为房颤持续时间超过36个月,另20例为窦性心律,以ELISA法检测血清HSP27/60/70含量,以Western斑点印迹法检测右心房心肌组织中HSP27/60/70的表达水平。
2.
The HSP27/60/70 level expression in right atrial samples was determined by Western-blot,the expression in serum samples was determined by ELISA.
所有患者手术均采用全麻体外循环,胸骨正中切口,体外循环开始前,取右心耳组织,立即置-70℃液氮保存,以Western斑点印迹法检测右心房心肌组织中HSP27/60/70含量,检测结果以积分光密度表示。
6) Dot blot
斑点印迹
1.
Application of DIFA to non-radioactive dot blot technique;
DIFA检测非放射性斑点印迹技术的探讨
2.
Methods: After the human esophageal cancer cells were cultured at final concentration of 10 -5 mol/L 8-Cl-cAMP for 24h, both the experimental group cells and the control group cells were identified by using intact cell RNA dot blot techniques.
方法 :原代分组培养食管癌细胞后 ,采用完整细胞原位RNA斑点印迹技术对滴膜进行斑点印迹。
3.
The expression of related gene and protein was studied by the intact cell RNA dot blot,in situ hybridization and protein dot blot.
方法 采用TUNEL法检测凋亡细胞 ,采用原位杂交和完整细胞原位斑点印迹技术检测凋亡相关基因及蛋白质的表达。
补充资料：斑点分析(spot analysis)
分子式：
CAS号：
性质：又称斑点分析(spot analysis)。在滤纸上进行的点滴试验。取小块滤纸，用毛细滴管点加试液和试剂，以形成带色斑点对试样进行定性鉴定。滤纸具有毛细吸附性，使试液浓集，可提高鉴定反应的灵敏度；由于滤纸对不同的离子吸附能力具有差异，吸附力强的集中在中心，吸附力弱的向外扩散。当在纸上进行混合物的分析时，可直接达到分离的目的，省却了在容器中进行分离的冗长手续。
CAS号：
性质：又称斑点分析(spot analysis)。在滤纸上进行的点滴试验。取小块滤纸，用毛细滴管点加试液和试剂，以形成带色斑点对试样进行定性鉴定。滤纸具有毛细吸附性，使试液浓集，可提高鉴定反应的灵敏度；由于滤纸对不同的离子吸附能力具有差异，吸附力强的集中在中心，吸附力弱的向外扩散。当在纸上进行混合物的分析时，可直接达到分离的目的，省却了在容器中进行分离的冗长手续。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条