1) Western blotting
Western印迹法
1.
Using western blotting and MTT chemosensi-tive testing, the relationship between the expression of NFkB family proteins, P53 and MDM2 in ovarian cancer cell lines and chemotherapy resistance of ovarian cell was ob.
培养13个卵巢癌细胞系,提取癌细胞蛋白,采用Western印迹法观察NFkB家族蛋白、P53和MDM2的表达,并用四氮唑盐法药敏试验观察与卵巢癌细胞化疗耐药性关系。
2.
Using the mouse antibody to Hsp70of human,pig,dog,etc,Hsp70was detected from seven randomly sele cted yeast strains by Western blotting.
随机抽取其中7株新生隐球菌,采用鼠抗人、猪、狗等的Hsp70抗体,通过Western印迹法来检测其Hsp70,根据实验信号强弱定性判断在不同刺激条件下的Hsp70产量。
3.
Using Western blotting and MTT chemosensitive testing, the relationship between the expression of NFκB family proteins in ovarian cancer cell lines with chemotherapy resistance of ovarian cell was observed.
方法 :培养 13株卵巢癌细胞系 ,对其进行胞质、胞核蛋白质提取 ,Western印迹法观察NFκB家族蛋白及其抑制物的表达 ,并用MTT检测药敏、观察NFκB家族蛋白与卵巢癌细胞化疗耐药性关系。
2) Western blot
Western印迹法
1.
Methods Western blot and immunohistochemistry were performed to detect the expression and phosphorylation status of GluR1.
方法采用Western印迹法、免疫组织化学法检测纹状体AMPA受体亚单位GluR1表达及磷酸化状态。
3) Far-Western blotting
Far-Western印迹法
4) Western-blot
Western斑点印迹法
1.
The HSP27/60/70 expression level in right atrial samples was determined by Western-blot,while the expression in serum samples was determined by enzyme-linked immunosorb.
方法选取瓣膜置换手术患者40例,其中20例为房颤持续时间超过36个月,另20例为窦性心律,以ELISA法检测血清HSP27/60/70含量,以Western斑点印迹法检测右心房心肌组织中HSP27/60/70的表达水平。
2.
The HSP27/60/70 level expression in right atrial samples was determined by Western-blot,the expression in serum samples was determined by ELISA.
所有患者手术均采用全麻体外循环,胸骨正中切口,体外循环开始前,取右心耳组织,立即置-70℃液氮保存,以Western斑点印迹法检测右心房心肌组织中HSP27/60/70含量,检测结果以积分光密度表示。
5) western blotting
蛋白质印迹(法)，Western印迹法
6) Western blotting
Western印迹
1.
METHODS: The mRNA level and protein expression of ANXA1 in some OSCC cell lines and human primary OSCC tissue specimens were detected using real-time PCR ,Western blotting and immunohistochemistry.
方法:应用实时定量PCR、Western印迹方法和免疫组化方法检测ANXA1在口腔鳞癌细胞株和30例原发口腔鳞癌中的表达,采用SPSS10。
2.
Methods The ES antigens (ESA) of Trichinella spiralis muscle larvae cultured in vitro at 18 h and 30 h were analyzed by SDS-PAGE and Western blotting.
方法 应用SDS PAGE和Western印迹对旋毛虫肌幼虫体外培养 18、3 0h后的ES抗原中的蛋白组分进行研究。
3.
9 breast cancer cell lines were cultured and cells harvested, protein of p50, p65 and IkB extracted from cytoplasm, nuclear separately to detect the expression of p50, p65, IkB proteins by Western blotting.
用 L-1 5培养液培养并传代 9个乳腺癌细胞系 ,收获细胞 ,提取胞浆、胞核蛋白质 ,Western印迹法观察 NFk B家族蛋白及其抑制物的表达 ;采用 2 0 J· m- 2 剂量紫外线照射细胞 ,继续培养细胞并提取蛋白质进行检测。
补充资料：“诺森”印迹法
分子式：
CAS号：
性质：又称RNA印迹（法）。与DNA斑迹法过程类似的技术。用于从凝胶中转移出RNA片段转移至硝酸纤维素膜，尼龙膜等，并用探针进行杂交（称“北方”杂交）以显示目的区段的位置。显示RNA的大小及丰度。
CAS号：
性质：又称RNA印迹（法）。与DNA斑迹法过程类似的技术。用于从凝胶中转移出RNA片段转移至硝酸纤维素膜，尼龙膜等，并用探针进行杂交（称“北方”杂交）以显示目的区段的位置。显示RNA的大小及丰度。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条