1) plasmid vector pUC18
质粒载体pUC18
1.
The target blunt-ended DNA was cloned into plasmid vector pUC18 which was previously digested with Sma I and dephosphorylated with calf alkaline phosphatase.
6~3 kb之间的DNA片段用T4 DNA聚合酶补平,再与SmaI酶切消化并经去磷酸化处理的质粒载体pUC18连接,转化至DH10B大肠杆菌(Escherichia coli)的感受态细胞中。
2) pUC18 carrier
pUC18质粒载体
1.
Then use T4 ligase to connect recycled DNA and pUC18 carrier.
以不吸水链霉菌梧州新亚种为材料,提取的总基因组经Sau3AI不完全酶切,回收3~9 kb DNA酶切片段,用T4连接酶将回收片段按一定比例与经BamHI酶切、南极热敏磷酸酶去磷酸化处理后的pUC18质粒载体连接,热激法转化受体菌E。
3) plasmid pUC18
质粒pUC18
4) Desoxyribose nucleic acid
PUC18质粒DNA
5) vector PUC18-HGF
PUC18-HGF载体
6) PUC18 cloning vector
PUC18克隆载体
补充资料：PUC18质粒DNA
CAS: 9007-49-2
中文名称: PUC18质粒脱氧核糖核酸；PUC18质粒DNA
英文名称: Deoxyribonucleic-acid；Calf thymus DNA；Desoxyribose nucleic acid；DNA；Nucleic acids, deoxyribo；deoxyribonucleic ac.；Nucleic acids,deoxyribo
中文名称: PUC18质粒脱氧核糖核酸；PUC18质粒DNA
英文名称: Deoxyribonucleic-acid；Calf thymus DNA；Desoxyribose nucleic acid；DNA；Nucleic acids, deoxyribo；deoxyribonucleic ac.；Nucleic acids,deoxyribo
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