1) plasmid vevtor
pSUPER质粒载体
2) pSUPER plasmid
pSUPER质粒
3) pSUPER-EGFP vector
pSUPER-EGFP载体
4) pSUPER vector
pSUPER载体
1.
pSUPER vector was linearized with BgLⅡ and HindⅢ treated with alkaline phosphatase(CIP).
方法:化学合成2段编码短发夹RNA序列的、靶向Smad3的寡核苷酸,各64个碱基,退火,克隆到经BgLⅡ和HindⅢ双酶切同时经牛小肠碱性磷酸酶(alkaline phosphatase,CIP)处理后的pSUPER载体的polyⅢH1启动子的下游,重组构建RNA干扰(RNA induced interference,RNAi)质粒,同时设立错义寡核苷酸作为非特异性对照。
5) plasmid
[英]['plæzmid] [美]['plæzmɪd]
质粒载体
1.
This study was aimed to investigate the effect of plasmid-mediated RNAi targeting hTERT on blocking hTERT gene and inhibiting telomerase activity in leukemia cell line K562.
本研究探讨质粒载体介导的RNAi靶向hTERT对白血病细胞株K562 hTERT基因封闭、端粒酶活性抑制的作用。
6) Plasmid vector
质粒载体
1.
Construction and identification of plasmid vector of RNA interference of transforming growth factor-β1 gene
转化生长因子-β1基因RNAi质粒载体的构建与鉴定
2.
A new plasmid vector pMD-Fib-IE-EGFP which contains enhanced green fluorescent protein gene as reportor gene was constructed by subtitute EGFP for GFP in the original plasmid pG350.
以含家蚕丝心蛋白重链基因同源片段的质粒pG35 0为出发质粒 ,插入增强型绿色荧光蛋白 (EGFP)基因 ,构建成以EGFP为报告基因的同源重组质粒载体 pMD Fib IE EGFP ,通过PCR及酶切鉴定表明EGFP以正确的方式插入到原始质粒中 ,通过脂质体介导法转染胃癌细胞能发出很强的绿色荧光 ,表明该质粒能够在真核细胞中表达 ,为进一步建立完善的家蚕转基因系统平台提供基因材
3.
Effective Production and Ex vivo Evaluation of Plasmid Vector Intended for Human Cancer Gene TherapyABSTRCTThe effective production of plasmid vector intended for gene therapy was investigated systematically.
本文较系统地研究了用于肿瘤基因治疗的质粒载体的高效制备工艺。
补充资料：PUC18质粒DNA
CAS: 9007-49-2
中文名称: PUC18质粒脱氧核糖核酸；PUC18质粒DNA
英文名称: Deoxyribonucleic-acid；Calf thymus DNA；Desoxyribose nucleic acid；DNA；Nucleic acids, deoxyribo；deoxyribonucleic ac.；Nucleic acids,deoxyribo
中文名称: PUC18质粒脱氧核糖核酸；PUC18质粒DNA
英文名称: Deoxyribonucleic-acid；Calf thymus DNA；Desoxyribose nucleic acid；DNA；Nucleic acids, deoxyribo；deoxyribonucleic ac.；Nucleic acids,deoxyribo
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
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