1) Plasmid vector
质粒载体
1.
Construction and identification of plasmid vector of RNA interference of transforming growth factor-β1 gene
转化生长因子-β1基因RNAi质粒载体的构建与鉴定
2.
A new plasmid vector pMD-Fib-IE-EGFP which contains enhanced green fluorescent protein gene as reportor gene was constructed by subtitute EGFP for GFP in the original plasmid pG350.
以含家蚕丝心蛋白重链基因同源片段的质粒pG35 0为出发质粒 ,插入增强型绿色荧光蛋白 (EGFP)基因 ,构建成以EGFP为报告基因的同源重组质粒载体 pMD Fib IE EGFP ,通过PCR及酶切鉴定表明EGFP以正确的方式插入到原始质粒中 ,通过脂质体介导法转染胃癌细胞能发出很强的绿色荧光 ,表明该质粒能够在真核细胞中表达 ,为进一步建立完善的家蚕转基因系统平台提供基因材
3.
Effective Production and Ex vivo Evaluation of Plasmid Vector Intended for Human Cancer Gene TherapyABSTRCTThe effective production of plasmid vector intended for gene therapy was investigated systematically.
本文较系统地研究了用于肿瘤基因治疗的质粒载体的高效制备工艺。
2) plasmid
[英]['plæzmid] [美]['plæzmɪd]
质粒载体
1.
This study was aimed to investigate the effect of plasmid-mediated RNAi targeting hTERT on blocking hTERT gene and inhibiting telomerase activity in leukemia cell line K562.
本研究探讨质粒载体介导的RNAi靶向hTERT对白血病细胞株K562 hTERT基因封闭、端粒酶活性抑制的作用。
4) plasmid vector pUC18
质粒载体pUC18
1.
The target blunt-ended DNA was cloned into plasmid vector pUC18 which was previously digested with Sma I and dephosphorylated with calf alkaline phosphatase.
6~3 kb之间的DNA片段用T4 DNA聚合酶补平,再与SmaI酶切消化并经去磷酸化处理的质粒载体pUC18连接,转化至DH10B大肠杆菌(Escherichia coli)的感受态细胞中。
5) pUC18 carrier
pUC18质粒载体
1.
Then use T4 ligase to connect recycled DNA and pUC18 carrier.
以不吸水链霉菌梧州新亚种为材料,提取的总基因组经Sau3AI不完全酶切,回收3~9 kb DNA酶切片段,用T4连接酶将回收片段按一定比例与经BamHI酶切、南极热敏磷酸酶去磷酸化处理后的pUC18质粒载体连接,热激法转化受体菌E。
6) plasmid DNA vector
质粒DNA载体
补充资料：PUC18质粒DNA
CAS: 9007-49-2
中文名称: PUC18质粒脱氧核糖核酸；PUC18质粒DNA
英文名称: Deoxyribonucleic-acid；Calf thymus DNA；Desoxyribose nucleic acid；DNA；Nucleic acids, deoxyribo；deoxyribonucleic ac.；Nucleic acids,deoxyribo
中文名称: PUC18质粒脱氧核糖核酸；PUC18质粒DNA
英文名称: Deoxyribonucleic-acid；Calf thymus DNA；Desoxyribose nucleic acid；DNA；Nucleic acids, deoxyribo；deoxyribonucleic ac.；Nucleic acids,deoxyribo
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