一步法实时定量RT-PCR,one-step real-time quantitative reverse transcriptase PCR,音标,读音,翻译,英文例句,英语词典

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1) one-step real-time quantitative reverse transcriptase PCR 点击朗读

一步法实时定量RT-PCR

In this study, a rapid and specific TaqMan-based, one-step real-time quantitative reverse transcriptase PCR (qRT-PCR) is developed for the detection of peste des petits ruminants virus (PPRV).

建立了检测小反刍兽疫病毒的快速、特异的基于Taqman的一步法实时定量RT-PCR方法。

2) one step real-time fluorescent RT-PCR 点击朗读

一步法实时荧光RT-PCR

For detection of BPMV in imported soybean,the specific primers and Taqman probe were designed based on the coding gene of BPMV coat protein and one step RT-PCR and one step real-time fluorescent RT-PCR methods were developed.

针对进口大豆的菜豆荚斑驳病毒(Bean pod mottle virus,BPMV)检测,建立了一步法RT-PCR和一步法实时荧光RT-PCR检测方法。

3) realtime PCR/RT-PCR 点击朗读

实时定量PCR/RT-PCR

4) Real-time RT-PCR with the external standard 点击朗读

外标准法实时定量RT-PCR

5) Real-time quantitative RT-PCR 点击朗读

荧光实时定量RT-PCR法

6) real-time RT-PCR 点击朗读

实时定量RT-PCR

The viruses from concentrated samples were detected by real-time RT-PCR. 点击朗读

向水样中接种已知量的脊髓灰质炎病毒,然后利用微孔滤膜吸附—洗脱法、滑石粉—硅藻土吸附层吸附—洗脱法、化学絮凝沉淀法和滑石粉—硅藻土絮凝沉淀法等4种不同的浓缩方法对水样中的病毒进行浓缩,并采用实时定量RT-PCR技术对各方法浓缩后样品中的病毒进行扩增和检测。

By means of real-time RT-PCR,microporous filters with various nominal pores and materials were compared;the membrane elution method was modified;the effect of PEG on virus recovery was studied;the optimal method was determined at last.

通过实时定量RT-PCR检测,比较了不同材料和不同孔径的微孔滤膜对病毒的吸附效果;对膜洗脱方式进行了改进;研究了在洗脱液浓缩过程中,PEG浓度对于病毒回收率的影响。

The pattern of murine endogenous retrovirus-like gene(MuERV-L)mRNA expression was examined in the early preimplantation development of mice,using real-time RT-PCR technique.

利用实时定量RT-PCR技术,对体外培养的小鼠植入前胚胎发育过程中鼠内源逆转录病毒样基因(MuERV-L)的表达进行研究,并通过appd icolin(DNA复制抑制剂)、TSA(组蛋白去乙酰化酶特异性抑制剂)分别抑制1-细胞期DNA的复制和合子基因组激活(ZGA)过程中组蛋白去乙酰化,探索MuERV-L基因表达的变化。

更多例句>>

补充资料：immune PCR

分子式：
CAS号：

性质：通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。

参考词条

荧光实时定量PCR(Q-RT-PCR) 一步法RT-PCR 实时荧光一步RT-PCR 实时RT-PCR法实时荧光定量RT-PCR

定量实时RT-PCR 实时荧光定量PCR(RT-FQ-PCR)

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	您的位置：首页 -> 词典 -> 一步法实时定量RT-PCR 1) one-step real-time quantitative reverse transcriptase PCR 一步法实时定量RT-PCR 1. In this study, a rapid and specific TaqMan-based, one-step real-time quantitative reverse transcriptase PCR (qRT-PCR) is developed for the detection of peste des petits ruminants virus (PPRV). 建立了检测小反刍兽疫病毒的快速、特异的基于Taqman的一步法实时定量RT-PCR方法。 2) one step real-time fluorescent RT-PCR 一步法实时荧光RT-PCR 1. For detection of BPMV in imported soybean,the specific primers and Taqman probe were designed based on the coding gene of BPMV coat protein and one step RT-PCR and one step real-time fluorescent RT-PCR methods were developed. 针对进口大豆的菜豆荚斑驳病毒(Bean pod mottle virus,BPMV)检测,建立了一步法RT-PCR和一步法实时荧光RT-PCR检测方法。 3) realtime PCR/RT-PCR 实时定量PCR/RT-PCR 4) Real-time RT-PCR with the external standard 外标准法实时定量RT-PCR 5) Real-time quantitative RT-PCR 荧光实时定量RT-PCR法 6) real-time RT-PCR 实时定量RT-PCR 1. The viruses from concentrated samples were detected by real-time RT-PCR. 向水样中接种已知量的脊髓灰质炎病毒,然后利用微孔滤膜吸附—洗脱法、滑石粉—硅藻土吸附层吸附—洗脱法、化学絮凝沉淀法和滑石粉—硅藻土絮凝沉淀法等4种不同的浓缩方法对水样中的病毒进行浓缩,并采用实时定量RT-PCR技术对各方法浓缩后样品中的病毒进行扩增和检测。 2. By means of real-time RT-PCR,microporous filters with various nominal pores and materials were compared;the membrane elution method was modified;the effect of PEG on virus recovery was studied;the optimal method was determined at last. 通过实时定量RT-PCR检测,比较了不同材料和不同孔径的微孔滤膜对病毒的吸附效果;对膜洗脱方式进行了改进;研究了在洗脱液浓缩过程中,PEG浓度对于病毒回收率的影响。 3. The pattern of murine endogenous retrovirus-like gene(MuERV-L)mRNA expression was examined in the early preimplantation development of mice,using real-time RT-PCR technique. 利用实时定量RT-PCR技术,对体外培养的小鼠植入前胚胎发育过程中鼠内源逆转录病毒样基因(MuERV-L)的表达进行研究,并通过appd icolin(DNA复制抑制剂)、TSA(组蛋白去乙酰化酶特异性抑制剂)分别抑制1-细胞期DNA的复制和合子基因组激活(ZGA)过程中组蛋白去乙酰化,探索MuERV-L基因表达的变化。更多例句>> 补充资料：immune PCR 分子式： CAS号：性质：通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。说明：补充资料仅用于学习参考，请勿用于其它任何用途。参考词条荧光实时定量PCR(Q-RT-PCR) 一步法RT-PCR 实时荧光一步RT-PCR 实时RT-PCR法实时荧光定量RT-PCR

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