1) One-step RT-PCR
一步法RT-PCR
1.
Detection of Citrus psorosis virus by one-step RT-PCR and its annual distribution in different parts of Dweet tangor;
运用一步法RT-PCR检测柑橘鳞皮病毒及其在橘橙不同部位中的全年分布
2.
Research and Application of One-step RT-PCR Kit in the Detection of Tobacco Ringspot virus;
一步法RT-PCR检测烟草环斑病毒试剂盒的研制与应用
3.
Development of a one-step RT-PCR assay for detection of A subtype avian influenza virus;
禽流感病毒一步法RT-PCR检测方法的建立
2) one step RT-PCR
一步法RT-PCR
1.
E2 gene was amplified by one step RT-PCR from total RNA,which had been extracted from tissues of pigs doubting infected by classical swine fever virus and PK-15 infected by CSFV F114.
自疑似猪瘟病料及接猪瘟F114毒的PK-15细胞中提取总RNA,经一步法RT-PCR扩增E2基因。
2.
For detection of BPMV in imported soybean,the specific primers and Taqman probe were designed based on the coding gene of BPMV coat protein and one step RT-PCR and one step real-time fluorescent RT-PCR methods were developed.
针对进口大豆的菜豆荚斑驳病毒(Bean pod mottle virus,BPMV)检测,建立了一步法RT-PCR和一步法实时荧光RT-PCR检测方法。
3) one step duplex RT-PCR
一步法复合RT-PCR
1.
Development of one step duplex RT-PCR technique for detection of H5 subtype avian influenza virus;
H5亚型禽流感病毒一步法复合RT-PCR检测方法的建立
4) one-step multiplex RT-PCR
一步法多重RT-PCR
1.
Simultaneous detection of porcine circovirus type 2 and porcine respiratory and reproductive syndrome virus by one-step multiplex RT-PCR;
一步法多重RT-PCR检测猪圆环病毒2型和猪繁殖与呼吸道综合征病毒
6) One-step RT-PCR
一步RT-PCR
1.
34 kb was amplified by one-step RT-PCR using the total RNA of the PVY infected plant;ho.
本研究依据PVY CP基因序列设计合成了一对引物PY1、PY2,以带毒样品植物总RNA为模板,在同一个反应中同时加入反转录和PCR反应所需试剂,反应程序中包括反转录和PCR反应所需条件,进行反应扩增,带毒样品扩增得到340 bp的目的条带,而健康对照无此目的条带,从而建立了PVY的一步RT-PCR检测技术,并组装成试剂盒。
2.
The expected product(335 bp) could be amplified by two-step RT-PCR and one-step RT-PCR from the infected samples,while no amplified products were obtained from the healthy tissue samples.
两种方法相比,一步RT-PCR法特异性强、灵敏度高、省时经济、程序简单,达到了快速检测的目的。
补充资料：immune PCR
分子式：
CAS号：
性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
CAS号：
性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条