1) Quantitative real-time reverse transcriptase-poly merase chain reaction
定量实时RT-PCR
2) realtime PCR/RT-PCR
实时定量PCR/RT-PCR
3) real-time RT-PCR
实时定量RT-PCR
1.
The viruses from concentrated samples were detected by real-time RT-PCR.
向水样中接种已知量的脊髓灰质炎病毒,然后利用微孔滤膜吸附—洗脱法、滑石粉—硅藻土吸附层吸附—洗脱法、化学絮凝沉淀法和滑石粉—硅藻土絮凝沉淀法等4种不同的浓缩方法对水样中的病毒进行浓缩,并采用实时定量RT-PCR技术对各方法浓缩后样品中的病毒进行扩增和检测。
2.
By means of real-time RT-PCR,microporous filters with various nominal pores and materials were compared;the membrane elution method was modified;the effect of PEG on virus recovery was studied;the optimal method was determined at last.
通过实时定量RT-PCR检测,比较了不同材料和不同孔径的微孔滤膜对病毒的吸附效果;对膜洗脱方式进行了改进;研究了在洗脱液浓缩过程中,PEG浓度对于病毒回收率的影响。
3.
The pattern of murine endogenous retrovirus-like gene(MuERV-L)mRNA expression was examined in the early preimplantation development of mice,using real-time RT-PCR technique.
利用实时定量RT-PCR技术,对体外培养的小鼠植入前胚胎发育过程中鼠内源逆转录病毒样基因(MuERV-L)的表达进行研究,并通过appd icolin(DNA复制抑制剂)、TSA(组蛋白去乙酰化酶特异性抑制剂)分别抑制1-细胞期DNA的复制和合子基因组激活(ZGA)过程中组蛋白去乙酰化,探索MuERV-L基因表达的变化。
4) real-time quantitative RT-PCR
实时定量RT-PCR
1.
The clinical value of real-time quantitative RT-PCR detection for fusion genes in leukemia;
实时定量RT-PCR技术检测白血病融合基因的临床应用价值
2.
Development and application of Taqman real-time quantitative RT-PCR assay for detection of taura syndrome virus in shrimps;
对虾Taura综合症病毒Taqman实时定量RT-PCR检测方法的建立与应用
3.
And expressions of TLR9 mRNA on peripheral blood,were detected by using real-time quantitative RT-PCR method.
选取HSV阳性样品50例,选取HSV阴性10例做为对照组;采用实时定量RT-PCR方法进行TLR9 mRNA检测。
5) real time quantitative RT-PCR
实时定量RT-PCR
1.
This paper introduced the principle, method and application of Real Time Quantitative RT-PCR which includes SYBR Green I, AmpliSensor, Lightcycle and Complex technologies, In Situ PCR and Degenerate PCR.
介绍了实时定量RT-PCR的原理、方法和应用,包括SYBR Green I、AmpliSensor、Lightcycle技术和Complex探针技术;同时还对原位PCR技术的原理、方法和应用,简并PCR的原理、方法和应用作了综述。
2.
To investigate the feasibility of using exogenous salicylic acid(SA) to regulate the allelopathic weed suppression of rice,this paper studied the effects of different concentrations exogenous SA on the weed-suppression and physiological-biochemical characteristics of allelopathic rice PI312777,and the relative expression quantity of gene ZB8 in the rice by real time quantitative RT-PCR(FQ-PCR).
为了探讨外源水杨酸(SA)调控水稻化感抑草效应的可行性,研究了不同浓度的外源SA对强化感水稻PI312777抑草效应的影响及其生理生化特性,并运用实时定量RT-PCR(FQ-PCR)技术检测SA介导的关键酶苯丙氨酸解氨酶基因(ZB8)的相对表达量。
6) Real time RT-PCR
实时定量RT-PCR
1.
The HOXB4 expression at mRNA level was assayed by using real time RT-PCR.
本研究采用实时定量RT-PCR的方法在mRNA水平上检测HOXB4基因的表达,以观察体外扩增脐血CD34+细胞自我更新的水平。
2.
To investigate the mechanism of action of anti-inflammatory and immunological regulation,the change of interleukin-2 gene in the level of mRNA in the spleen of healthy and immunosuppressive mouse was detected with the technology of real time RT-PCR.
采用实时定量RT-PCR技术检测正常小鼠和免疫抑制模型小鼠脾脏内白介素-2(IL-2)基因mRNA水平表达量的变化。
补充资料：immune PCR
分子式：
CAS号：
性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
CAS号：
性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条