1) Hn-1/06 strain
河南分离株Hn-1/06株
1.
Five pair of specific primers of PRRSV ORF3 to ORF7 were designed and synthesized according to the published gene sequence of PRRSV from GenBank(PRU87392),and ORF3,ORF4,ORF5,ORF6,ORF7 genes of PRRSV Hn-1/06 strain were obtained by RT-PCR method.
参照GenBank中公布的猪繁殖与呼吸综合征病毒(PRRSV)美洲株ATCC VR2332的ORF3~7基因序列,利用Pri mer软件分别设计合成了针对PRRSV ORF3、ORF4、ORF5、ORF6和ORF7基因的特异性引物,利用RT-PCR从河南分离株Hn-1/06株分别扩增得到了大小约964、675、718、566和501 bp的片段,并将扩增的片段插入PGEM-T-easy载体进行测序。
2.
Cloning and Expression of N Gene of PRRSV Hn-1/06 Strain and Establishment of Indirect ELISA Based on the Recombinant Fusion Protein;
本研究利用原核表达系统对PRRSV河南分离株Hn-1/06株N蛋白基因进行了高效可溶性表达,并用重组的N蛋白作为抗原,成功地建立了检测PRRSV血清抗体的N蛋白间接ELISA方法。
3) Henan isolates
河南分离株
1.
Variation analysis of the ORF5 gene of porcine reproductive and respiratory syndrome virus(PRRSV)of Henan isolates;
猪繁殖与呼吸综合征病毒河南分离株ORF5基因的克隆与变异分析
4) HN strain
HN株
5) HeBei strain
河北分离株
1.
A strain of egg drop syndrome virus (EDSV) was detected from chicks which suffered from egg drop syndrome (EDS) in HeBei and provisionally named as HeBei strain (EDSV-HB).
本研究主要对减蛋综合征病毒河北分离株(EDSV-HB)的六邻体(Hexon)基因进行了扩增、克隆、测序、表达及免疫原性的研究。
6) DN-1 strain
DN-1分离株
1.
It was amplified by RT-PCR from IBV DN-1 strain.
2%,表明IBVDN-1分离株与疫苗株的S1基因具有高度的同源性。
补充资料：赤株
1.花叶萎败的株干。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条