1) suppression subtractive hybridization
抑制扣除杂交
1.
To identify differentially expressed genes between fetal mesenchymal stem cell (MSC) and adult MSC, especially specified genes expressed in fetal MSC, a cDNA subtractive library of fetal MSC was constructed using suppression subtractive hybridization (SSH) technique.
为了获得胎儿和成人间充质干细胞 (mesenchymalstemcells,MSC)的差异表达基因 ,尤其是在胎儿MSC中特异表达的基因 ,应用抑制扣除杂交技术构建了胎儿和成人MSCcDNA扣除文库。
3) suppression subtractive hybridization (SSH)
抑制性扣除杂交(SSH)
1.
Identification of Nitrate-induced genes by Suppression Subtractive Hybridization (SSH) and Their Expression Pattern Analysis in Rice Root;
利用抑制性扣除杂交（SSH）技术克隆水稻根系硝态氮诱导基因及诱导基因表达谱的研究
4) subtractive hybridization
扣除杂交
1.
Separation of gene clones related to crocin synthesis using subtractive hybridization;
用扣除杂交法分离藏红花苷合成相关基因的克隆
2.
Methods The differentially expressed genes were cloned by modified PCR-based subtractive hybridization.
方法利用聚合酶链反应改良扣除杂交技术,克隆正常组与腭裂组小鼠腭突组织中差异表达的基因,经反向点杂交鉴定后挑选阳性克隆并测序及同源性分析,Northern印迹杂交检测所获的新基因mRNA的全长。
3.
The technique,principle,advantage and disadvantage of several mRNA diffrential display means, such as differential screening,subtractive hybridization,reveise transcription PCR, representational difference analysis,suppression subtractive hybridization and its application in rice molecular biology.
对mRNA差异显示法中人们比较熟悉的示差筛选、扣除杂交和DDRT PCR法 ,以及近几年新提出的RDA、SSH 5种主要的差异基因筛选法 ,从其原理、在分子生物学中的应用、优缺点及其改进方法等方面进行了简要概
5) solid subtractive hybridization
固相扣除杂交
1.
Direct cloning of differential expression genes with full-length by a modified solid subtractive hybridization.;
一种改进的固相扣除杂交法直接克隆全长差异表达基因
6) suppression subtractive hybridization(SSH)
抑制消减杂交
1.
Application of suppression subtractive hybridization(SSH) to study of cold tolerance in common carp(Cyprinus carpio);
抑制消减杂交技术在鲤鱼抗寒研究中的应用
2.
Differentially expressed cDNAs subtracted female from male gametophytes of Laminaria japonica were obtained by suppression subtractive hybridization(SSH).
利用抑制消减杂交法(suppression subtractive hybridization,SSH)获得了海带雌、雄配子体差异表达的cDNA片段,将雄配子体差异表达的cDNA片段克隆于pGEM-T载体并转化大肠杆菌(Escherichia coli)JM109,构建雄配子体差异表达的cDNA消减文库。
3.
The subtractive cDNA libraries for RH strain(typeⅠ) and QHO strain(typeⅡ) were constructed by the technique of suppression subtractive hybridization(SSH) using the PCR-Select cDNA Subtraction Kit.
用抑制消减杂交技术(SSH)构建弓形虫强弱毒株正向消减(弱毒株消减强毒株)及反向消减(强毒株消减弱毒株)cDNA文库。
补充资料：抑制杂交翻译
分子式：
CAS号：
性质：又称杂交扣留翻译。能使一克隆DNA与它所编码的蛋白质相关联的方法。这个方法基于下述事实：以RNA在与它的DNA互补体杂交后就不会在无细胞的翻译系统中指导蛋白质的合成。无细胞系统所合成的蛋白质，包括一个特定的杂交mRNA，就能与未发生同DNA杂交的系统所合成的蛋白质相比较，其差别就表示了由所研究的mRNA编码的蛋白质的性质。
CAS号：
性质：又称杂交扣留翻译。能使一克隆DNA与它所编码的蛋白质相关联的方法。这个方法基于下述事实：以RNA在与它的DNA互补体杂交后就不会在无细胞的翻译系统中指导蛋白质的合成。无细胞系统所合成的蛋白质，包括一个特定的杂交mRNA，就能与未发生同DNA杂交的系统所合成的蛋白质相比较，其差别就表示了由所研究的mRNA编码的蛋白质的性质。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条