1) Suppression subtractive hybridization
抑制消减杂交法
1.
Suppression subtractive hybridization (SSH) method was employed to analyze the differently expressed cDNA sequence between the strained VSMCs pretreated with and without wortmannin, a specific inhibitor of PI3K.
方法应用FX-4000T细胞应变加载系统,对大鼠主动脉VSMCs施加1Hz、10%张应变,Western blotting检测细胞在不同加载时间下Akt磷酸化水平的变化;用抑制消减杂交法(SSH),筛选给予PI3K的抑制剂Wortmannin和给予DMSO两组细胞在加载张应变24h后的差异表达基因片段,得到的消减杂交产物连接到T载体上,经过蓝白斑筛选,对所有阳性克隆进行菌液PCR筛选及测序,应用BLASTN进行序列比对。
2) suppression subtractive hybridization(SSH)
抑制消减杂交
1.
Application of suppression subtractive hybridization(SSH) to study of cold tolerance in common carp(Cyprinus carpio);
抑制消减杂交技术在鲤鱼抗寒研究中的应用
2.
Differentially expressed cDNAs subtracted female from male gametophytes of Laminaria japonica were obtained by suppression subtractive hybridization(SSH).
利用抑制消减杂交法(suppression subtractive hybridization,SSH)获得了海带雌、雄配子体差异表达的cDNA片段,将雄配子体差异表达的cDNA片段克隆于pGEM-T载体并转化大肠杆菌(Escherichia coli)JM109,构建雄配子体差异表达的cDNA消减文库。
3.
The subtractive cDNA libraries for RH strain(typeⅠ) and QHO strain(typeⅡ) were constructed by the technique of suppression subtractive hybridization(SSH) using the PCR-Select cDNA Subtraction Kit.
用抑制消减杂交技术(SSH)构建弓形虫强弱毒株正向消减(弱毒株消减强毒株)及反向消减(强毒株消减弱毒株)cDNA文库。
3) suppression subtractive hybridization
抑制性消减杂交
1.
Screening and cloning of target genes transactivated by human gene C1 encoding protein 1 interacting with HBV core antigen using suppression subtractive hybridization technique;
抑制性消减杂交技术筛选乙型肝炎病毒核心蛋白相互作用蛋白编码基因C1的反式调节基因
2.
Construction of cDNA library of hepatitis B virus with X protein C-terminally truncated 40 amino acids by suppression subtractive hybridization method;
抑制性消减杂交构建乙型肝炎病毒X蛋白羧基端缺失40个氨基酸cDNA文库
3.
Identification of genes associated with the metastasis of adenoid cystic carcinoma by suppression subtractive hybridization;
应用抑制性消减杂交技术分析涎腺腺样囊性癌转移相关基因
4) SSH
抑制性消减杂交
1.
Screening Correlative Genes from Citrinin Biosynthetic Pathway in Monascus Species of by SSH;
应用抑制性消减杂交法筛选与红曲菌产桔霉素相关的基因
2.
Methods:Suppression Subtractive Hybridization(SSH) was performed to profile differentially express genes between normal abortive fetus (as the driver) and Down s syndrome fetus(as the tester),then screening and analyzing the subtractive libraries.
方法:利用抑制性消减杂交的方法对DS胎儿及正常胎儿脑组织样品进行正向消减杂交,利用斑点杂交对所获的基因片断进行进一步的验证。
3.
coli132(UPEC132)by suppression subtractive hybridization(SSH).
目的:应用抑制性消减杂交技术,构建致肾盂肾炎大肠埃希菌132株(UPEC132)与已完成基因组测序的非致病性大肠埃希菌标准株MG1655的基因组DNA消减文库。
5) suppression subtractive hybridization
抑制消减杂交
1.
Suppression subtractive hybridization coupled with gene chip technologies and their application;
抑制消减杂交与基因芯片技术及其联合应用
2.
Cloning of differentially expressed genes of eosinophils from asthmatic patients by suppression subtractive hybridization;
应用抑制消减杂交克隆支气管哮喘病人嗜酸细胞差异表达基因
3.
Suppression subtractive hybridization in construction of radiation-induced EST library;
运用抑制消减杂交构建辐射诱导EST库
6) suppressive subtractive hybridization
抑制消减杂交
1.
The suppressive subtractive cDNA library was set up using suppressive subtractive hybridization(SSH).
方法提取胰腺癌和癌旁正常胰腺组织中的RNA和mRNA,应用抑制消减杂交技术(SSH)构建消减cDNA文库。
补充资料：抑制杂交翻译
分子式：
CAS号：
性质：又称杂交扣留翻译。能使一克隆DNA与它所编码的蛋白质相关联的方法。这个方法基于下述事实：以RNA在与它的DNA互补体杂交后就不会在无细胞的翻译系统中指导蛋白质的合成。无细胞系统所合成的蛋白质，包括一个特定的杂交mRNA，就能与未发生同DNA杂交的系统所合成的蛋白质相比较，其差别就表示了由所研究的mRNA编码的蛋白质的性质。
CAS号：
性质：又称杂交扣留翻译。能使一克隆DNA与它所编码的蛋白质相关联的方法。这个方法基于下述事实：以RNA在与它的DNA互补体杂交后就不会在无细胞的翻译系统中指导蛋白质的合成。无细胞系统所合成的蛋白质，包括一个特定的杂交mRNA，就能与未发生同DNA杂交的系统所合成的蛋白质相比较，其差别就表示了由所研究的mRNA编码的蛋白质的性质。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条