1) suppression subtractive hybridization
抑制差减杂交
1.
In order to study the genetic differences between epidemic and nonepidemic strains of Vibrio choleraeO1 E1 Tor, the suppression subtractive hybridization was used to screen for the different genes between epidemic and nonepidemic strains of V.
为了克隆与分析霍乱弧菌O1E1Tor流行株与非流行株两类菌株基因组差异片段 ,采用抑制差减杂交技术 (suppressionsubtractivehybridization ,SSH)分别以国内保留的流行株Wujiang 2及非流行株Js 32一株作为被检菌 ,另一株作为参考菌进行基因组差异研究 。
2.
To research salt tolerance mechanism and get the salt tolerance relative genes of Eustoma grandiflorum, a cDNA library was made by suppression subtractive hybridization technique.
为了研究草原龙胆抗盐性机理,初步获得其抗盐性相关基因,分别以400mmol·L-1的NaCl及清水处理的草原龙胆叶片mRNA为tester和driver方,利用抑制差减杂交技术构建了cDNA文库。
3.
Suppression subtractive hybridization (SSH) was carried out by using resistant pool as tester and susceptible pool as driver and SSH DNA library was constructed.
以YW642/中8601的F2代纯合的抗病和感病单株各10株提取DNA构建成近等基因池,以抗病池为试验方,以感病池为驱动方,进行抑制差减杂交(SSH),构建差减文库。
2) SSH
抑制差减杂交
1.
Isolation of Genes with Enhanced Expressions in Rice Treated with Probenazole Using SSH Technique;
用抑制差减杂交技术分离烯丙异噻唑诱导水稻特异表达的基因
2.
Principle and manipulation result analysis for suppression subtractive hybridization(SSH);
抑制差减杂交技术原理及常见操作结果分析
3.
To understand the gene expression characteristics,which involved in drought tolerance,five cDNA libraries were constructed using suppression subtractive hybridization(SSH) method with wheat seedlings sampled at 1,6,12,24 and 48 hours of water stress,respectively.
利用抑制差减杂交技术,分别构建小麦幼苗在水分胁迫1 h、6 h、12 h2、4 h和48 h条件下的cDNA文库,得到6 733条EST序列。
3) Suppression subtractive hybridization (SSH)
抑制差减杂交
1.
) Hook], a forward subtractive cDNA library was constructed using suppression subtractive hybridization (SSH) method, which was performed using the cDNA from the mutant Dugansha clone as the tester and the cDNA from the normal Jurong 0 clone as the driver.
为了获得杉木木质化过程中特异表达的基因,本研究利用抑制差减杂交技术,以杉木突变体独干杉无性系为测试方(Tester),正常的句容0号无性系为驱动方(Driver),构建了正向差减文库,获得了618个克隆。
2.
Suppression subtractive hybridization (SSH) was utilized for the isolation of cDNA fragments for Euonymus japonicus ‘Zhuzi’ differentially expressed genes, and forward suppression subtractive cDNA Library of cold-regulated gene was constructed.
应用抑制差减杂交(suppressionsubtractivehybridization,SSH)方法,构建冷诱导表达的正向抑制差减cDNA文库,低温处理的幼苗为tester,常温处理为driver,通过cDNA宏阵列差异筛选cDNA文库,得到604个低温诱导或表达增强的候选克隆,对其中的84克隆进行DNA测序,去除冗余的cDNA,在GenBank中进行核酸和蛋白质同源性的比较和功能分析,共有36个单一序列,有12个cDNA未找到同源序列,可能为新基因,表明采用抑制差减杂交方法与cDNA宏阵列技术分离诱导表达的基因是可行的。
3.
To investigate the expression profile of maize genes induced by submergence, a subtracted cDNA library of maize seedling roots was constructed using suppression subtractive hybridization (SSH).
)幼苗根部cDNA为目标群体 ,未处理 (untreated ,UT)的玉米幼苗根部cDNA为对照群体 ,进行抑制差减杂交。
4) suppression subtractive hybridization(SSH)
抑制差减杂交
1.
A suppression subtractive hybridization(SSH) cDNA library was constructed with an incompatible combination between wheat(Triticum aestivum) cultivar Xingzi 9104 and Puccinia striiformis f.
以成株期抗条锈小麦(Triticum aestivum)品种兴资9104为材料,依照CLONTECH公司PCR-SelectTMcDNA Subtraction Kit方法构建了小麦成株期条锈菌(Puccinia striiformis)诱导的抑制差减杂交(suppression subtractive hybridization,SSH)cDNA文库。
2.
To clone the differential expressional genes between the pre-metamorphosis(17 day post hatching,DPH) and pro-metamorphosis stage(23 DPH),we used suppression subtractive hybridization(SSH) to construct a cDNA library of pro-metamorphosis flounder Paralichthys olivaceus,when the RNA from pre-metamorphosis larvae was used as the driver.
为了克隆变态早期牙鲆头部差异表达的基因,采用抑制差减杂交法,以变态前的仔鱼头部表达的RNA作为驱动RNA,建立了牙鲆变态早期的差减cDNA文库,并利用相对定量RT-PCR对其进行了筛选。
5) suppression subtractive hybridization
抑制性差减杂交
1.
Suppression subtractive hybridization(SSHs) was performed to distinguish the differentially expressed genes.
取培养48h的2组细胞提取总RNA后分离mRNA,应用抑制性差减杂交法分离差异表达基因,并连接于pGEM TEasyVector,转化大肠杆菌JM109,构成差减cDNA文库,经蓝白斑筛选后挑选白色菌落,提取质粒,序列测定后进行同源性比较分析。
2.
A systematic study was initiated to identify differentially expressed genes in the process of sex reversal of the grouper by using suppression subtractive hybridization (SSH) technique.
应用抑制性差减杂交 (SSH)技术构建了石斑鱼性反转前后性腺组织的SMARTcDNA文库及其cDNA差减文库 ,从中随机挑取 12 0 0个克隆进行了PCR和斑点杂交筛选 ,得到 12 0个差异表达cDNA片段 。
3.
Objective: To Construct a subtracted cDNA library of hyperlipidemia sensitive rabbits′liver by suppression subtractive hybridization(SSH).
结论:用抑制性差减杂交及T/A克隆技术成功构建了高脂血症敏感兔差异表达基因差减cDNA文库。
6) suppression subtractive hybridization(SSH)
抑制差减杂交(SSH)
补充资料：抑制杂交翻译
分子式：
CAS号：
性质：又称杂交扣留翻译。能使一克隆DNA与它所编码的蛋白质相关联的方法。这个方法基于下述事实：以RNA在与它的DNA互补体杂交后就不会在无细胞的翻译系统中指导蛋白质的合成。无细胞系统所合成的蛋白质，包括一个特定的杂交mRNA，就能与未发生同DNA杂交的系统所合成的蛋白质相比较，其差别就表示了由所研究的mRNA编码的蛋白质的性质。
CAS号：
性质：又称杂交扣留翻译。能使一克隆DNA与它所编码的蛋白质相关联的方法。这个方法基于下述事实：以RNA在与它的DNA互补体杂交后就不会在无细胞的翻译系统中指导蛋白质的合成。无细胞系统所合成的蛋白质，包括一个特定的杂交mRNA，就能与未发生同DNA杂交的系统所合成的蛋白质相比较，其差别就表示了由所研究的mRNA编码的蛋白质的性质。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条