1) relative quantitative real time RT-PCR
荧光实时相对定量RT-PCR
1.
In this research,the small interfering RNAs(siRNAs) obtained by in vitro transcriptional methods were applied to suppress the expression of chTERT mRNA in MDCC-MSB1 cells by relative quantitative real time RT-PCR,and to evaluate the biological consequences of chTERT down-regulation on M.
为通过体外转录的小干扰RNA(Small interference RNA,siRNA)抑制马立克氏病淋巴瘤细胞(MDCC-MSB1)中鸡端粒酶反转录酶(Chicken telomerase reverse transcriptase,chTERT)mRNA的表达,探讨chTERT在MDCC-MSB1中的作用,本试验设计了针对chTERT mRNA的特异siRNA序列(siRNA-1、siRNA-2、siRNA-3),利用T7 RNA聚合酶体外转录系统,制备21 bp的siRNA分子,通过脂质体介导转染MDCC-MSB1细胞,采用荧光实时相对定量RT-PCR方法检测转染细胞chTERT基因mRNA的相对表达量,流式细胞仪测定转染细胞周期变化情况。
2) RT-FQ-PCR
实时荧光定量PCR(RT-FQ-PCR)
3) quantitative real-time PCR(Q-RT-PCR)
荧光实时定量PCR(Q-RT-PCR)
4) real-time fluorescent quantitative RT-PCR
实时荧光定量RT-PCR
1.
Expressions of bone metastasis genes in nasopharyngeal carcinoma by real-time fluorescent quantitative RT-PCR;
实时荧光定量RT-PCR检测鼻咽癌骨转移相关基因表达的研究
2.
Establishment of a real-time fluorescent quantitative RT-PCR assay for the detection of chicken IFN-γ gene;
鸡γ-干扰素实时荧光定量RT-PCR检测方法的建立
3.
Methods Using real-time fluorescent quantitative RT-PCR to analyze the expressions of DNMT1,DNMT3A and DNMT3B in normal liver cell line,pericacinoma cell line and hepatocellular carcinoma cell line respectively.
方法用实时荧光定量RT-PCR方法分析正常肝细胞系、肝癌癌旁细胞系及肝癌癌细胞系中DNMT1、DNMT3A、DNMT3B的表达谱,以及DNMT3B表达抑制后在肝癌细胞系SMMC-7721中出现表达上调的与肿瘤发生相关的基因PDCD4和MBD3的表达。
5) Real-time fluorescence quantitative RT-PCR
实时荧光定量RT-PCR
1.
Quantification of enterovirus 71 by real-time fluorescence quantitative RT-PCR in hospitalized children with hand-foot-mouth disease
实时荧光定量RT-PCR法检测手足口病患儿大便标本中肠道病毒71型
2.
Methods After treatment of cultured 16HBE cells with NiSO_4 or NiSO_4 adding FJD drug serum,the variation of the free radicals and 8-oxo-dGTPase expression were detected by laser scanning confocal microscopy(LSCM) and real-time fluorescence quantitative RT-PCR,respectively.
方法体外培养的16HBE细胞分别经NiSO4暴露及NiSO4加扶正解毒汤含药血清共同处理后,采用激光扫描共聚焦显微镜(LSCM)及实时荧光定量RT-PCR检测自由基含量及8-oxo-dGTPase表达的变化。
3.
Conclusion Real-time fluorescence quantitative RT-PCR used to detect the expression of .
目的建立实时荧光定量RT-PCR方法检测SurvivinmRNA的表达。
6) real time RT-PCR
实时荧光定量RT-PCR
1.
One step RT-PCR technique and Real time RT-PCR technique are applied in evaluating the correlation between rate of mRNA degradation in SD rats and postmortem intervals, especially in advanced stage PMI.
目的：本研究从基因库中筛选出两种稳定表达于不同个体与组织细胞中的看家基因GAPDH和β-actin，采用实时荧光定量RT-PCR技术研究大鼠死后不同时间和不同脏器中两种看家基因的mRNA降解规律，建立mRNA降解的程度与死亡时间关系的回归方程，探索用于晚期死亡时间推断较为理想的组织，并试图建立标准化的RNA提取与定量检测方法，希望能最终应用于法医学实践中。
补充资料：高压荧光灯用荧光粉
分子式：
CAS号：
性质：高压荧光灯中涂上Eu3+激活的钒酸钇或钒磷酸钇(YVO4:Eu，Y(V，P)O4:Eu)，可提高光效，改善显色性，增加了灯中的红色比和显色指数。YVO4白色立方晶体耐温度猝灭，发光效率高，传递效率大，高压荧光汞灯主要用于街道，厂房和场地照明。
CAS号：
性质：高压荧光灯中涂上Eu3+激活的钒酸钇或钒磷酸钇(YVO4:Eu，Y(V，P)O4:Eu)，可提高光效，改善显色性，增加了灯中的红色比和显色指数。YVO4白色立方晶体耐温度猝灭，发光效率高，传递效率大，高压荧光汞灯主要用于街道，厂房和场地照明。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条