1)  pre-column derivatizing method
柱前衍生化法
2)  pre-column derivatization
柱前衍生化法
3)  Pre-column derivatization
柱前衍生
1.
Determination of Tauine by internal standard HPLC with pre-column derivatization;
柱前衍生高效液相色谱内标法检测牛磺酸的研究
2.
Determination of Cyclamate in Children Foods by HPLC with Pre-Column Derivatization;
柱前衍生液相色谱测定儿童食品中甜蜜素
3.
Process diagnosis for reduction reaction of 2-aminoethansulfonic processing by HPLC pre-column derivatization;
HPLC柱前衍生法2-氨基乙磺酸制备工艺还原阶段流程诊断
4)  Precolumn derivatization
柱前衍生
1.
Determination of amino acids in food by High Performance Liquid Chromatography with precolumn derivatization;
柱前衍生高效液相色谱法测定食品中的氨基酸含量
2.
Determination of fenfluramine in slimming drug by HPLC with precolumn derivatization using 2,4-dinitrofluorobenzene as derivative reagent;
硝基氟苯柱前衍生HPLC测定减肥药中芬氟拉明
3.
Determination of sodium valproate in serum by HPLC after precolumn derivatization;
改进的柱前衍生高效液相色谱法测定人血中丙戊酸钠
5)  derivatization
柱前衍生
1.
The separation with quantitative analytical methodof taurine in Pinctada martensii was studied by high performance liquid chromatographic (HPLC) after derivatization with opthaldialdehyde.
应用邻苯二甲醛柱前衍生高效液相色谱法,测定了马氏珠母贝中牛磺酸,结果表明:采用邻苯二甲醛为柱前衍生剂,与原料中的牛磺酸发生衍生化反应后,以甲醇-乙酸钠溶液(体积比40︰60)为流动相,使用ODSC18分离柱,FLD检测器(EX=338nm,EM=444nm)等色谱条件下检测,牛磺酸的线性范围为2。
2.
A novel fluorescence reagent 1,2-benzo-3,4-dihydrocarbazole-9-ethyl-toluene-4-sulfonate (BDBETS) was synthesized, and using which as pre-column derivatization reagent ,19 fatty acids were derivatized optimally.
合成了新型荧光试剂 1,2_苯并_3,4_二氢咔唑_9_乙基对甲苯磺酸酯 ,以此作为柱前衍生化试剂对19种游离脂肪酸 (FFA)进行了优化衍生 。
3.
A new method for the separation of amino acids by capillary electrophores is used and 9_(2_carbazole)ethyl chloroformate(CEOC) as derivatization is developed.
采用新合成的荧光衍生试剂咔唑＿9＿乙基氯甲酸酯(CEOC)作为柱前衍生试剂,利用毛细管电泳仪对游离氨基酸进行了分离,研究了该试剂用于氨基酸分离的几个关键的条件,从而实现了对14种氨基酸的基线分离。
6)  pre-column derivation
柱前衍生
1.
Determination of gamma-aminobutyric acid and seventeen free amino acids in Pinus massoniana Needles by reversed-phase high performance liquid chromatography with pre-column derivation;
柱前衍生反相高效液相色谱法测定马尾松(Pinus massoniana)针叶中γ-氨基丁酸和17种游离氨基酸
2.
That taurine in milk powder was determined by HPLC using pre-column derivation by 2,4-dinitrofluorobenzene (DNFB).
建立了用 2 ,4 二硝基氟苯作为柱前衍生试剂 ,采用高效液相色谱仪 ,紫外检测器成功检测出乳粉中牛磺酸含量的方法。
3.
A method for measurement of L-leucine by high pertormance liquid chromatographic(HPLC)and orthophaladetyde(OPA) pre-column derivation are described.
研究采用高效液相色谱(HPLC)和邻苯二甲醛(OPA)柱前衍生法测定L-亮氨酸的条件。
参考词条
补充资料：柱前衍生化法
分子式：
CAS号：

性质：被测组分先通过衍生化反应，转化为衍生化产物，然后再经过色谱柱进行分离。高效液相色谱法中柱前衍生的目的是：通过衍生化反应，使原来不能直接被检测的组分转化为能被所用检测器检测的物质；使被测组分与衍生化试剂有选择地参加反应，而与样品中的其他组分分离；改变被测组分在色谱柱上的出峰次序，使之更有利于分离。柱前衍生的优点是可以不必严格限制衍生化反应条件，可以允许较长的反应时间及使用各种形式的反应器。其缺点是当一个复杂组分样品经过衍生化反应后，有可能产生多种衍生化产物，给色谱分离带来困难。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。