1)  precolumn derivatization
柱前衍生剂
2)  Pre-column derivatization
柱前衍生
1.
Determination of Tauine by internal standard HPLC with pre-column derivatization;
柱前衍生高效液相色谱内标法检测牛磺酸的研究
2.
Determination of Cyclamate in Children Foods by HPLC with Pre-Column Derivatization;
柱前衍生液相色谱测定儿童食品中甜蜜素
3.
Process diagnosis for reduction reaction of 2-aminoethansulfonic processing by HPLC pre-column derivatization;
HPLC柱前衍生法2-氨基乙磺酸制备工艺还原阶段流程诊断
3)  Precolumn derivatization
柱前衍生
1.
Determination of amino acids in food by High Performance Liquid Chromatography with precolumn derivatization;
柱前衍生高效液相色谱法测定食品中的氨基酸含量
2.
Determination of fenfluramine in slimming drug by HPLC with precolumn derivatization using 2,4-dinitrofluorobenzene as derivative reagent;
硝基氟苯柱前衍生HPLC测定减肥药中芬氟拉明
3.
Determination of sodium valproate in serum by HPLC after precolumn derivatization;
改进的柱前衍生高效液相色谱法测定人血中丙戊酸钠
4)  derivatization
柱前衍生
1.
The separation with quantitative analytical methodof taurine in Pinctada martensii was studied by high performance liquid chromatographic (HPLC) after derivatization with opthaldialdehyde.
应用邻苯二甲醛柱前衍生高效液相色谱法,测定了马氏珠母贝中牛磺酸,结果表明:采用邻苯二甲醛为柱前衍生剂,与原料中的牛磺酸发生衍生化反应后,以甲醇-乙酸钠溶液(体积比40︰60)为流动相,使用ODSC18分离柱,FLD检测器(EX=338nm,EM=444nm)等色谱条件下检测,牛磺酸的线性范围为2。
2.
A novel fluorescence reagent 1,2-benzo-3,4-dihydrocarbazole-9-ethyl-toluene-4-sulfonate (BDBETS) was synthesized, and using which as pre-column derivatization reagent ,19 fatty acids were derivatized optimally.
合成了新型荧光试剂 1,2_苯并_3,4_二氢咔唑_9_乙基对甲苯磺酸酯 ,以此作为柱前衍生化试剂对19种游离脂肪酸 (FFA)进行了优化衍生 。
3.
A new method for the separation of amino acids by capillary electrophores is used and 9_(2_carbazole)ethyl chloroformate(CEOC) as derivatization is developed.
采用新合成的荧光衍生试剂咔唑＿9＿乙基氯甲酸酯(CEOC)作为柱前衍生试剂,利用毛细管电泳仪对游离氨基酸进行了分离,研究了该试剂用于氨基酸分离的几个关键的条件,从而实现了对14种氨基酸的基线分离。
5)  pre-column derivation
柱前衍生
1.
Determination of gamma-aminobutyric acid and seventeen free amino acids in Pinus massoniana Needles by reversed-phase high performance liquid chromatography with pre-column derivation;
柱前衍生反相高效液相色谱法测定马尾松(Pinus massoniana)针叶中γ-氨基丁酸和17种游离氨基酸
2.
That taurine in milk powder was determined by HPLC using pre-column derivation by 2,4-dinitrofluorobenzene (DNFB).
建立了用 2 ,4 二硝基氟苯作为柱前衍生试剂 ,采用高效液相色谱仪 ,紫外检测器成功检测出乳粉中牛磺酸含量的方法。
3.
A method for measurement of L-leucine by high pertormance liquid chromatographic(HPLC)and orthophaladetyde(OPA) pre-column derivation are described.
研究采用高效液相色谱(HPLC)和邻苯二甲醛(OPA)柱前衍生法测定L-亮氨酸的条件。
6)  pre column derivatization
柱前衍生
1.
A novel fluorescence and ultraviolet labelling reagent, 2 (9 carbazole) ethyl chloroformate, has been utilized as pre column derivatization reagent for 15 aliphatic alcohols.
采用新型的紫外、荧光衍生试剂咔唑 9 乙基氯甲酸酯 ,对 15种脂肪醇柱前衍生的反应条件 ,包括反应温度、催化剂的选择、衍生化时间等 ,进行了考察。
2.
This paper reports a reversed phase high performance liquid chromatographic method for the simultaneous separation and determination of histamine(Hist) and noradrenaline(NA) in human plasma by means of pre column derivatization of Hist and NA with OPA in the presence of 2 mercaptoethanol.
用邻苯二甲醛（ＯＰＡ）进行柱前衍生，流动相为Ｖ（乙酸钠缓冲溶液）Ｖ（乙腈）＝７３，检测器电位＋０。
3.
A high performance liquid chromatographic pre column derivatization method with fluorescence detection for the analysis of glutamine(Gln) in rat intestinal mucosa is presented.
，５μｍ）柱上，研究并建立了测定动物肠粘膜中谷氨酰胺（Ｇｌｎ）的柱前衍生荧光ＲＰ－ＨＰＬＣ法。
参考词条
补充资料：柱前衍生化法
分子式：
CAS号：

性质：被测组分先通过衍生化反应，转化为衍生化产物，然后再经过色谱柱进行分离。高效液相色谱法中柱前衍生的目的是：通过衍生化反应，使原来不能直接被检测的组分转化为能被所用检测器检测的物质；使被测组分与衍生化试剂有选择地参加反应，而与样品中的其他组分分离；改变被测组分在色谱柱上的出峰次序，使之更有利于分离。柱前衍生的优点是可以不必严格限制衍生化反应条件，可以允许较长的反应时间及使用各种形式的反应器。其缺点是当一个复杂组分样品经过衍生化反应后，有可能产生多种衍生化产物，给色谱分离带来困难。

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