1) RT PCR
RT-PCR
1.
RT PCR was then performed.
目的建立灵敏外周血人乳房珠蛋白(hMAM)表达 RT-PCR 检测方法,探索 hMAM 在乳腺癌诊治中的应用价值。
2.
IgM positive tissues from 12 spontaneous abortions were studied by RT PCR for the data .
方法用ELISA法检测749例有异常妊娠史的育龄妇女和305例正常对照组妇女RV抗体RV-IgM、IgG,同时检测其中361例自然流产少于3次和52例自然流产3次以上的育龄妇女,并结合RT-PCR技术对12例IgM阳性的自然流产组织进行检测,数据行卡方统计。
3.
It was used in the RT PCR to amplify DEN RNA extracted from DEN infected mosquitos.
方法利用标记地高辛(Dig)的登革病毒通用引物进行RT-PCR扩增蚊媒体内的病毒RNA,然后以反向斑点杂交法检测病毒扩增片段并进行分型。
2) reverse transcription-polymerase chain reaction
RT-PCR
1.
With beta-actin gene as the internal standard,reverse transcription-polymerase chain reaction(RT-PCR) was used to examine the expression of fms-like tyrosine kinase(Flt-1) mRNA in endometrium of Small Tail Han sheep and Northeast Fine-wool sheep.
以β-actin基因作为内源性内标,采用RT-PCR方法检测了Flt-1基因mRNA在东北细毛羊、小尾寒羊妊娠期不同阶段子宫内膜中的表达量。
2.
MethodsThe expression of FEZ1 mRNA in 50 cases of esophageal carcinoma and 50 cases of normal esophageal mucoma were detected by reverse transcription-polymerase chain reaction(RT-PCR).
方法采用RT-PCR法检测50例食管鳞癌及50例正常食管组织中FEZ1 mRNA的表达情况,分析其与食管鳞癌患者各临床病理指标的关系。
3.
Methods The mRNA of Ki-67 and VEGF were detected using reverse transcription-polymerase chain reaction(RT-PCR) in 55 RCC tissues and 20 normal renal tissues.
方法应用半定量RT-PCR技术检测55例肾癌组织和20例正常肾组织Ki-67和VEGFmRNA表达情况。
3) Reverse transcription polymerase chain reaction
RT-PCR
1.
Then the level of VEGF mRNA in kidney was determined by reverse transcription polymerase chain reaction (RT-PCR); the expression of VEGF and VEGF.
【方法】取3月龄、12月龄大鼠作为对照组,24月龄大鼠作为观察组各6只,提取其肾脏组织的RNA,应用逆转录聚合酶链反应技术(RT-PCR)检测肾脏组织内VEGFmRNA转录水平,以及应用免疫组化技术检测肾小球VEGF和VEGFR蛋白的表达;应用酶联免疫法检测肾组织培养液的VEGF总含量。
2.
Methods Reverse transcription polymerase chain reaction (RT PCR) was used to evaluate the amount of insulin like growth factor mRNA in exercise induced damaged skeletal muscle, and specimens were used for ultrastructural observation.
方法利用IGF引物对不同损伤程度骨骼肌标本进行RT-PCR检测,同时观查损伤骨骼肌超微结构改变。
3.
Methods NIT-1 cells were exposed to cyclosporin A (10 μmol/L) for 24 and 48 h respectively, after which the amount of insulin release was determined by means of radioimmunoassay (RIA), and the differential expressions of Nuox23, Cox7c and Atp5K genes assessed by semi-quantitative reverse transcription polymerase chain reaction.
放射免疫测定法(RIA)检测胰岛素释放,半定量RT-PCR检测CsA处理NIT-1胰岛β细胞后线粒体氧化磷酸化酶系中的几种主要成员(Nuox23、Cox7c和Atp5K)在mRNA水平上的表达。
4) RTPCR
RT-PCR
1.
Expression of cbfa1 gene in rat osteosarcoma UMR106 cell was detected by RTPCR after the 72?h s treatment of FSK88 liquid with different mol concentration(25,50,100?μmol/L),Retinoic Acid(RA,10?μmol/L) and mixed liquid of FSK88 and RA(1∶1).
以大鼠成骨肉瘤细胞———UMR106为模型,应用RT-PCR方法,检测用不同浓度的FSK88药液(25,50,100μmol/L)、维甲酸(RA,10μmol/L,阳性对照)以及FSK88+RA(等体积)混合药液处理细胞72 h后,细胞内cbfa1基因表达量的变化。
2.
by RTPCR and 3′-RACE technology.
]实生苗为材料,采用RT-PCR及RACE技术,获得了PPF-1(开花、衰老相关基因)同源基因的cDNA全长序列PtPPF-1,该cDNA全长1 493 bp,编码452个氨基酸,与豌豆PPF-1、拟南芥ALB3、水稻PPF-1及葡萄一未命名基因的氨基酸序列同源性较高,说明PPF-1基因在进化过程中可能变异较小。
5) RT PCR
RTPCR
6) real time RT-PCR
实时RT-PCR
1.
To establish the real time RT-PCR method to measure the expression of c-erbB-2 mRNA in breast cancer tissues of beside tumors and tissues of adenosis of mammary glands, to find out the relationship of c-erbB-2 protein.
建立LightCycler实时RT-PCR方法检测乳腺癌、癌旁组织及乳腺腺病组织c-erbB-2基因mRNA的表达。
补充资料：immune PCR
分子式：
CAS号：
性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
CAS号：
性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条