By cross breeding,the maize pepc gene in the pepc transgenic rice was successfully incorporated into the parents of two-,three-line hybrid rice,including sterile lines (Peiai64S,2302S,2304S,2306S and Shuangjiu A) and restorer lines (5129,02428 and Wanjing97) to breed the high-photosynthetic efficiency parents of hybrid rice and utilize heterosis between C_4 and C_4/C_3 rice.

A full-length napin promoter sequence1 (138 bp) and a conserved region (181 bp) of PEPC gene were amplified from the genomic DNA of Brassica napus L.

Only one of the T-DNAs carried the pepc gene, while the other T-DNA carried the selectable marker gene hpt (confers resistance to hygromycin) and the β-glucuron.

Using an agrobacterium mediated transformation system,the intact gene of maize phosphoenolpyruvate carboxylase ( pepc ) were introducted into rice variety "Kitaake" and the transgenic rice plant with high level expression of pepc gene was obtained.

The quantitative competitive polymerase chain reaction (QC-PCR) system of using 35S promoter heterologous template for the detection of genetically modified maize was developed in this study.

According to the plasmid map of Genetically Modified Maize,we select exogenous genes(CaMV35S promoter,NOS terminator,PAT gene,IVS2/PAT,CDPK/CryIA(b),Maize genome/CaMV35S,PAT/CaMV35S!,Cry9C/CaMV35S!) and endogenous gene(IVR) as target genes,design and synthesize their primers.

Gene flow of transgenic corn to cultivated relatives in China;

Reviewing the controversial reports of ecological safety of transgenic corn published in 《Nature》;

The concentration of Cry1Ab insecticidal protein in different tissues of the Asian corn borer,Ostrinia furnacalis,feeding on Bt-transgenic corn were determined by enzyme-linked immunosorbent assay(ELISA).