PCR on Detection and Identification of GM Maize MON810;

The quantitative competitive polymerase chain reaction (QC-PCR) system of using 35S promoter heterologous template for the detection of genetically modified maize was developed in this study.

According to the plasmid map of Genetically Modified Maize,we select exogenous genes(CaMV35S promoter,NOS terminator,PAT gene,IVS2/PAT,CDPK/CryIA(b),Maize genome/CaMV35S,PAT/CaMV35S!,Cry9C/CaMV35S!) and endogenous gene(IVR) as target genes,design and synthesize their primers.

Gene flow of transgenic corn to cultivated relatives in China;

Reviewing the controversial reports of ecological safety of transgenic corn published in 《Nature》;

The concentration of Cry1Ab insecticidal protein in different tissues of the Asian corn borer,Ostrinia furnacalis,feeding on Bt-transgenic corn were determined by enzyme-linked immunosorbent assay(ELISA).

Generation of vector backbone-free and selectable marker-free transgenic maize (Zea mays L.) via ovary-drip method

We investigated the inheritance and expression of cry1A gene in transgenic maize ( Zea mays L.

A transgenic maize variety M2 carrying the Bar gene was planted to study the gene flow.

A primarily designed DNA chip coupled with the multiplex PCR system was also used to detect mixed GM maize samples.