1) metal affinity chromatography
金属鏊合亲和层析
1.
Methods: The recombinant protein expressed by IPTG in Escherichia coli was induced, the proteins purified by the metal affinity chromatography and they were detected by Western blot.
方法:在大肠杆菌中对目的蛋白进行诱导表达,并用金属鏊合亲和层析法进行分离纯化,经WesternBlot试验鉴定。
2) Immobilized metal ion affinity chromatography
金属螯合亲和层析
1.
Purification of recombinant hepcidin fusion protein by immobilized metal ion affinity chromatography;
重组Hepcidin融合蛋白的金属螯合亲和层析纯化
2.
Purification of anti-HBcAg monoclonal antibodies using immobilized metal ion affinity chromatography
金属螯合亲和层析法纯化抗乙肝核心抗原单克隆抗体
3.
Immobilized metal ion affinity chromatography(IMAC) is one of the most efficient techniques in biological macromolecular separation and purification which has the advantages of high specificity, high recovery efficiency, high purity and single-step operation.
金属螯合亲和层析具有螯合介质制备简单,吸附容量大,选择性及通用性较好,易于再生,成本低等优点,是分离纯化蛋白质等生物工程产品最有效的技术之一。
3) metal chelate affinity chromatography
金属螯合亲和层析
1.
Superoxide dismutase(SOD) was preliminarily separated from maize by precipitation with ammonium sulfate and gelfiltration and then purified by metal chelate affinity chromatography(MCAC).
利用硫酸铵分级沉淀、凝胶过滤初步分离了玉米SOD,利用金属螯合亲和层析纯化了SOD。
2.
Isolation and purification of two SOD components(A and B) from maize by metal chelate affinity chromatography and DEAE-cellulose ion-exchange chromatography and determination of their qualities had been carried out in this experiment.
利用金属螯合亲和层析和DEAE-纤维素离子交换层析相结合的方法分离纯化玉米SOD,得到2种达到电泳纯的SOD组分(A、B),并对它们的基本性质进行了初步分析。
4) Immobilized metal ion affinity chromatograph(IMAC)
金属螯合亲和层析
1.
By means of Immobilized metal ion affinity chromatograph(IMAC),comparing the results of different elution methods,a optimum purification route was attained.
采用金属螯合亲和层析法对十聚体脑钠素进行了纯化 ,比较了咪唑洗脱法和降pH值洗脱法对目标蛋白的纯化效果 ,得到了一条纯化目标蛋白最佳亲和层析纯化路线 ,为后续实验打下了很好的基
5) metal-chelated affinity chromatography
螯合金属亲和层析
6) Metal chelation chromatography
金属亲和层析
补充资料：鏊战
1.鏖战。鏊﹐通"鏖"。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条