1) Metal-chelated affinity
金属螯合亲和
1.
Recombinant human-like collagen with a hexahistidine tail attacked to the carboxyl end could be purified by Metal-chelated affinity adsorption.
本研究对利用金属螯合亲和方法纯化重组类人胶原蛋白的吸附条件进行了优化。
2) IMAC
金属螯合亲和色谱
1.
Preparation and evaluation of a novel solid phase in immobilized metal affinity chromatography(IMAC);
一种新型金属螯合亲和色谱固定相的制备及评价
3) Immobilized metal ion affinity chromatography
金属螯合亲和层析
1.
Purification of recombinant hepcidin fusion protein by immobilized metal ion affinity chromatography;
重组Hepcidin融合蛋白的金属螯合亲和层析纯化
2.
Purification of anti-HBcAg monoclonal antibodies using immobilized metal ion affinity chromatography
金属螯合亲和层析法纯化抗乙肝核心抗原单克隆抗体
3.
Immobilized metal ion affinity chromatography(IMAC) is one of the most efficient techniques in biological macromolecular separation and purification which has the advantages of high specificity, high recovery efficiency, high purity and single-step operation.
金属螯合亲和层析具有螯合介质制备简单,吸附容量大,选择性及通用性较好,易于再生,成本低等优点,是分离纯化蛋白质等生物工程产品最有效的技术之一。
4) metal chelate affinity chromatography
金属螯合亲和层析
1.
Superoxide dismutase(SOD) was preliminarily separated from maize by precipitation with ammonium sulfate and gelfiltration and then purified by metal chelate affinity chromatography(MCAC).
利用硫酸铵分级沉淀、凝胶过滤初步分离了玉米SOD,利用金属螯合亲和层析纯化了SOD。
2.
Isolation and purification of two SOD components(A and B) from maize by metal chelate affinity chromatography and DEAE-cellulose ion-exchange chromatography and determination of their qualities had been carried out in this experiment.
利用金属螯合亲和层析和DEAE-纤维素离子交换层析相结合的方法分离纯化玉米SOD,得到2种达到电泳纯的SOD组分(A、B),并对它们的基本性质进行了初步分析。
5) Immobilized metal ion affinity chromatograph(IMAC)
金属螯合亲和层析
1.
By means of Immobilized metal ion affinity chromatograph(IMAC),comparing the results of different elution methods,a optimum purification route was attained.
采用金属螯合亲和层析法对十聚体脑钠素进行了纯化 ,比较了咪唑洗脱法和降pH值洗脱法对目标蛋白的纯化效果 ,得到了一条纯化目标蛋白最佳亲和层析纯化路线 ,为后续实验打下了很好的基
6) metal-chelated affinity chromatography
螯合金属亲和层析
补充资料:金属
分子式:
CAS号:
性质:具有特有的金属光泽以及良好导电导热性的固态或液态单质。在固态时还具有延展性。大多数元素是金属元素,其特性由元素原子结构和晶体内部结构决定:原子电负性小,吸引电子能力不大,聚集状态时电子流动性大;价层s、p电子少,常形成密堆集晶体结构,如立方面心密堆集、六方密堆集及体心立方堆砌结构。
CAS号:
性质:具有特有的金属光泽以及良好导电导热性的固态或液态单质。在固态时还具有延展性。大多数元素是金属元素,其特性由元素原子结构和晶体内部结构决定:原子电负性小,吸引电子能力不大,聚集状态时电子流动性大;价层s、p电子少,常形成密堆集晶体结构,如立方面心密堆集、六方密堆集及体心立方堆砌结构。
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