1) Asymmetric PCR
不对称PCR
1.
Detection of Hepatitis B Virus Using Molecular Beacon and Asymmetric PCR;
分子信标用于不对称PCR检测乙型肝炎病毒
2.
Methods Different ways of sample preparation were observed based on HLA-A biochip, such as two different genome DNA extraction methods (Phenol-chloroform method and leucocyte lysis method), nano-magnetic beads (Fe 2O 3) used in nucleus cell separation, the primers concentration and ratio in asymmetric PCR and three PCR product purification ways.
方法 观察了样品制备方法 (传统酚 氯仿法、白细胞裂解液法、Fe2 O3 纳米磁珠 )、不对称PCR制备ssDNA的参数、PCR产物纯化方法对杂交结果的影响。
3.
By means of asymmetric PCR and indirect fl.
采用不对称PCR和间接荧光标记技术进行单链DNA扩增和荧光标记,标记样品与寡核苷酸芯片杂交后,进行芯片清洗、扫描及结果分析。
2) asymmetry PCR
不对称PCR
1.
Two different PCR methods,seminested symmetry PCR and seminested asymmetry PCR,were adopted respectively.
分别采用对称PCR和不对称PCR扩增,均可得到扩增的目的片段。
3) thermal asymmetric interlaced PCR (TAIL-PCR)
热不对称PCR(TAIL-PCR)
1.
oryzicola and the critical pathogen of bacterial leaf streak in rice (Oryza sativa), thermal asymmetric interlaced PCR (TAIL-PCR) and Tn5 transposon rescue were adopted to verify 8 virulence-reduced mutants screened from the Tn5 inserted library of the pathogen based on virulence assay in rice.
为快速准确地鉴定Tn5的插入位点和相应的功能基因,研究采用热不对称PCR(TAIL-PCR)和Tn5转座子质粒拯救两种技术,鉴别了在筛选水稻条斑病菌(Xanthomonas oryzaepv。
4) asymmetric PCR-SSCP
不对称PCR-SSCP
1.
Application of asymmetric PCR-SSCP in gene mutation detecting;
不对称PCR-SSCP在基因突变检测中的应用
2.
Applying the asymmetric PCR-SSCP combined with sequencing techniques,the intron3 of the polym orphism of H-FABP gene in WZS pig was analyzed in the trial based on WZS pigs and the correlation between intron3 and growth traits(body weight,body height,body length and chest circumference) was analyzed as well.
以海南五指山猪为试验材料,采用不对称PCR-SSCP和测序相结合技术,对海南五指山猪心脏脂肪酸结合蛋白(H-FABP)基因第3内含子(intron 3)进行多态检测,并分析其与生长性状(体重、体高、体长、胸围)的相关性。
5) touchdown asymmetric PCR
touchdown不对称PCR
6) Asymmetric reverse transcription polymerase chain reaction (RT-PCR)
不对称RT-PCR
1.
Asymmetric reverse transcription polymerase chain reaction (RT-PCR) and microarrays were combined to distinguish four virus including Avian influenza virus (AIV), Newcastle disease virus (NDV), Infectious bronchitis virus (IBV), and Infectious bursal disease virus (IBDV) and hemagglutinin (HA) subtypes H5, H7, and H9, and neuraminidase (NA) subtypes N1 and N2 of AIV.
本研究建立了一种基因芯片结合不对称RT-PCR鉴别诊断禽流感(Avian influenza,AI)及其主要亚型(H5, H7, H9和N1,N2)、鸡传染性支气管炎(Infectious bronchitis,IB)、新城疫(Newcastle disease,ND)、鸡传染性法氏囊(Infectious bursal disease,IBD)的基因芯片检测方法。
补充资料：对称与非对称
反映客观事物在结构、功能、时空上的特殊联系的范畴。对称指事物以一定的中介进行某种变化时出现的不变性，非对称指事物以一定的中介进行某种变化时出现的可变性。在自然界中普遍存在，形式多样。对称有空间对称(包括形象对称和结构对称)、时间对称、概念对称等。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条