1) thermal asymmetric interlaced PCR(TAIL-PCR)
热不对称嵌套PCR
2) thermal asymmetric interlaced PCR (TAIL-PCR)
热不对称PCR(TAIL-PCR)
1.
oryzicola and the critical pathogen of bacterial leaf streak in rice (Oryza sativa), thermal asymmetric interlaced PCR (TAIL-PCR) and Tn5 transposon rescue were adopted to verify 8 virulence-reduced mutants screened from the Tn5 inserted library of the pathogen based on virulence assay in rice.
为快速准确地鉴定Tn5的插入位点和相应的功能基因,研究采用热不对称PCR(TAIL-PCR)和Tn5转座子质粒拯救两种技术,鉴别了在筛选水稻条斑病菌(Xanthomonas oryzaepv。
3) TAIL-PCR
热不对称交错PCR
1.
TAIL-PCR is a powerful tool for the recovery of DNA fragment adjacent to known sequences.
热不对称交错PCR(TAIL-PCR)方法已广泛应用于从多种生物克隆已知DNA序列的侧翼序列。
2.
At the same time, the CBHⅠgene of exoglucanase was cloned and the eukaryotic expression vector was constructed by the molecular biology techniques such as RT-PCR, degenerate PCR and TAIL-PCR.
在已克隆的CBHⅠ基因片段的基础上,试验设计了随机兼并引物和特异性引物,以cDNA为模板,采用改良后的热不对称交错PCR(TAIL-PCR)技术分别成功克隆了该基因的3’端和5’端侧翼序列。
3.
TAIL-PCR:A Simple and Efficient Method to Isolate DNA Segments Adjacent to Known Sequence;
在分子生物学研究中,基因克隆和分子杂交的探针制备等操作常需分离与已知DNA序列邻近的未知序列,热不对称交错PCR(简称TAIL PCR)反应技术能够较好地解决上述难题。
4) Asymmetric PCR
不对称PCR
1.
Detection of Hepatitis B Virus Using Molecular Beacon and Asymmetric PCR;
分子信标用于不对称PCR检测乙型肝炎病毒
2.
Methods Different ways of sample preparation were observed based on HLA-A biochip, such as two different genome DNA extraction methods (Phenol-chloroform method and leucocyte lysis method), nano-magnetic beads (Fe 2O 3) used in nucleus cell separation, the primers concentration and ratio in asymmetric PCR and three PCR product purification ways.
方法 观察了样品制备方法 (传统酚 氯仿法、白细胞裂解液法、Fe2 O3 纳米磁珠 )、不对称PCR制备ssDNA的参数、PCR产物纯化方法对杂交结果的影响。
3.
By means of asymmetric PCR and indirect fl.
采用不对称PCR和间接荧光标记技术进行单链DNA扩增和荧光标记,标记样品与寡核苷酸芯片杂交后,进行芯片清洗、扫描及结果分析。
5) asymmetry PCR
不对称PCR
1.
Two different PCR methods,seminested symmetry PCR and seminested asymmetry PCR,were adopted respectively.
分别采用对称PCR和不对称PCR扩增,均可得到扩增的目的片段。
6) asymmetric PCR-SSCP
不对称PCR-SSCP
1.
Application of asymmetric PCR-SSCP in gene mutation detecting;
不对称PCR-SSCP在基因突变检测中的应用
2.
Applying the asymmetric PCR-SSCP combined with sequencing techniques,the intron3 of the polym orphism of H-FABP gene in WZS pig was analyzed in the trial based on WZS pigs and the correlation between intron3 and growth traits(body weight,body height,body length and chest circumference) was analyzed as well.
以海南五指山猪为试验材料,采用不对称PCR-SSCP和测序相结合技术,对海南五指山猪心脏脂肪酸结合蛋白(H-FABP)基因第3内含子(intron 3)进行多态检测,并分析其与生长性状(体重、体高、体长、胸围)的相关性。
补充资料：嵌套PCR
分子式：
CAS号：
性质：后一次PCR的引物互补于前一次PCR所扩增序列的内侧。
CAS号：
性质：后一次PCR的引物互补于前一次PCR所扩增序列的内侧。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条